Molecular pathways involved in loss of kidney graft function with tubular atrophy and interstitial fibrosis

Daniel Maluf, Valeria Mas, Kellie J. Archer, Kenneth Yanek, Eric M. Gibney, Anne L. King, Adrian Cotterell, Robert A. Fisher, Marc P. Posner

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Loss of kidney graft function with tubular atrophy (TA) and interstitial fibrosis (IF) causes most kidney allograft losses. We aimed to identify the molecular pathways involved in IF/TA progression. Kidney biopsies from normal kidneys (n = 24), normal allografts (n = 6), and allografts with IF/TA (n = 17) were analyzed using high-density oligonucleotide microarray. Probe set level tests of hypotheses tests were conducted to identify genes with a significant trend in gene expression across the three groups using Jonckheere-Terpstra test for trend. Interaction networks and functional analysis were used. An unsupervised hierarchical clustering analysis showed that all the IF/TA samples were associated with high correlation. Gene ontology classified the differentially expressed genes as related to immune response, inflammation, and matrix deposition. Chemokines (CX), CX receptor (for example, CCL5 and CXCR4), interleukin, and interleukin receptor (for example, IL-8 and IL10RA) genes were overexpressed in IF/TA samples compared with normal allografts and normal kidneys. Genes involved in apoptosis (for example, CASP4 and CASP5) were importantly overexpressed in IF/TA. Genes related to angiogenesis (for example, ANGPTL3, ANGPT2, and VEGF) were downregulated in IF/TA. Genes related to matrix production-deposition were upregulated in IF/TA. A distinctive gene expression pattern was observed in IF/TA samples compared with normal allografts and normal kidneys. We were able to establish a trend in gene expression for genes involved in different pathways among the studied groups. The top-scored networks were related to immune response, inflammation, and cell-to-cell interaction, showing the importance of chronic inflammation in progressive graft deterioration.

Original languageEnglish (US)
Pages (from-to)276-285
Number of pages10
JournalMolecular Medicine
Volume14
Issue number5-6
DOIs
StatePublished - May 1 2008

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Atrophy
Fibrosis
Transplants
Kidney
Allografts
Genes
Inflammation
Gene Expression
Interleukin Receptors
Gene Ontology
Interleukins
Oligonucleotide Array Sequence Analysis
Interleukin-8
Chemokines
Cell Communication
Vascular Endothelial Growth Factor A
Cluster Analysis
Down-Regulation
Apoptosis
Biopsy

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

Molecular pathways involved in loss of kidney graft function with tubular atrophy and interstitial fibrosis. / Maluf, Daniel; Mas, Valeria; Archer, Kellie J.; Yanek, Kenneth; Gibney, Eric M.; King, Anne L.; Cotterell, Adrian; Fisher, Robert A.; Posner, Marc P.

In: Molecular Medicine, Vol. 14, No. 5-6, 01.05.2008, p. 276-285.

Research output: Contribution to journalArticle

Maluf, D, Mas, V, Archer, KJ, Yanek, K, Gibney, EM, King, AL, Cotterell, A, Fisher, RA & Posner, MP 2008, 'Molecular pathways involved in loss of kidney graft function with tubular atrophy and interstitial fibrosis', Molecular Medicine, vol. 14, no. 5-6, pp. 276-285. https://doi.org/10.2119/2007-00111.Maluf
Maluf, Daniel ; Mas, Valeria ; Archer, Kellie J. ; Yanek, Kenneth ; Gibney, Eric M. ; King, Anne L. ; Cotterell, Adrian ; Fisher, Robert A. ; Posner, Marc P. / Molecular pathways involved in loss of kidney graft function with tubular atrophy and interstitial fibrosis. In: Molecular Medicine. 2008 ; Vol. 14, No. 5-6. pp. 276-285.
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