Molecular regulation of apoptosis in fast plantaris muscles of aged rats

Emidio E. Pistilli, Parco M. Siu, Stephen Alway

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

This study tested the hypothesis that aging exacerbates apoptotic signaling in rat fast plantaris muscle during muscle unloading. Plantaris muscle mass was 22% lower in aged animals and the apoptotic index was 600% higher, when compared to those in young adult animals. Following 14 days of hind-limb unloading, absolute plantaris muscle mass was 20% lower in young adult animals with a corresponding 200% higher elevation of the apoptotic index. Unloading had no affect on muscle weight or apoptotic index of aged plantaris muscles. The changes in proapoptotic messenger RNA (mRNA) for apoptotic protease activating factor-1 (Apaf-1), Bax, and inhibitor of differentiation protein-2 (Id2) were exacerbated with aging. Bax and Bcl-2 protein levels were also altered differently in aged muscle, compared to young. Significant positive correlations were observed between the changes in Id2 and Bax mRNA, and Id2 and caspase-9 mRNA. These data suggest that a pro-apoptotic environment may contribute to aging-associated atrophy in fast skeletal muscle, but apoptotic signaling differs by age.

Original languageEnglish (US)
Pages (from-to)245-255
Number of pages11
JournalJournals of Gerontology - Series A Biological Sciences and Medical Sciences
Volume61
Issue number3
DOIs
StatePublished - Jan 1 2006
Externally publishedYes

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Inhibitor of Differentiation Protein 2
Skeletal Muscle
Apoptosis
Muscles
Messenger RNA
Young Adult
Apoptotic Protease-Activating Factor 1
Caspase 9
Atrophy
Extremities
Weights and Measures
Proteins

All Science Journal Classification (ASJC) codes

  • Aging
  • Geriatrics and Gerontology

Cite this

Molecular regulation of apoptosis in fast plantaris muscles of aged rats. / Pistilli, Emidio E.; Siu, Parco M.; Alway, Stephen.

In: Journals of Gerontology - Series A Biological Sciences and Medical Sciences, Vol. 61, No. 3, 01.01.2006, p. 245-255.

Research output: Contribution to journalArticle

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