Monoclonal antibodies against human anti-F(ab′)2 antibodies react with light chain epitopes

Franco Silvestris, Nynke Rots, W. Bruce Yancey, Chris Malone, Robert Searles, Alan Solomon, Franco Dammacco, Ralph C. Williams

Research output: Contribution to journalArticle

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Abstract

Anti-F(ab′)2 antibodies affinity isolated from sera of patients with rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), or normal SLE relatives were used to produce monoclonal antibodies (mAbs) in Balb c and NZB mice. Four of five mAbs showed only primary light chain specificity. Only one mAb produced in an NZB mouse against anti-F(ab′)2 from a single SLE patient showed anti-μ-chain specificity. Parallel identical control immunizations with IgG or a single human IgG κ myeloma produced mAbs with a predominant γ-chain Fc fragment specificity. Anti-light chain specificity of mAbs was demonstrated to involve epitopes requiring tertiary structure of the entire light chain instead of antigens confined to C κ λ or V κ λ fragments. Anti-κ specificity of three mAbs was extremely similar but not identical to that defined by anti-Kml allotyping systems. No evidence was obtained with any of the mAbs produced for antigens unique to SLE or RA anti-F(ab′)2 antibodies. The light chain antigenic prominence of many anti-F(ab′)2 antibodies may reflect structural features shared by this group of immunoglobulins some-how important for their biologic function.

Original languageEnglish (US)
Pages (from-to)139-155
Number of pages17
JournalClinical Immunology and Immunopathology
Volume59
Issue number1
DOIs
StatePublished - Jan 1 1991

Fingerprint

Epitopes
Monoclonal Antibodies
Light
Systemic Lupus Erythematosus
Antibodies
Inbred NZB Mouse
Rheumatoid Arthritis
Immunoglobulin G
Immunoglobulin Fc Fragments
Antigens
Antibody Affinity
Immunoglobulins
Immunization
Serum

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Pathology and Forensic Medicine
  • Immunology

Cite this

Monoclonal antibodies against human anti-F(ab′)2 antibodies react with light chain epitopes. / Silvestris, Franco; Rots, Nynke; Yancey, W. Bruce; Malone, Chris; Searles, Robert; Solomon, Alan; Dammacco, Franco; Williams, Ralph C.

In: Clinical Immunology and Immunopathology, Vol. 59, No. 1, 01.01.1991, p. 139-155.

Research output: Contribution to journalArticle

Silvestris, F, Rots, N, Yancey, WB, Malone, C, Searles, R, Solomon, A, Dammacco, F & Williams, RC 1991, 'Monoclonal antibodies against human anti-F(ab′)2 antibodies react with light chain epitopes', Clinical Immunology and Immunopathology, vol. 59, no. 1, pp. 139-155. https://doi.org/10.1016/0090-1229(91)90088-R
Silvestris, Franco ; Rots, Nynke ; Yancey, W. Bruce ; Malone, Chris ; Searles, Robert ; Solomon, Alan ; Dammacco, Franco ; Williams, Ralph C. / Monoclonal antibodies against human anti-F(ab′)2 antibodies react with light chain epitopes. In: Clinical Immunology and Immunopathology. 1991 ; Vol. 59, No. 1. pp. 139-155.
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