Monoclonal antibodies directed against rat tracheal epithelial cells transformed in vitro

Gary R. Braslawsky, Stephen Kennel, R. E. Hand, Paul Nettesheim

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Cloned hybridoma cell lines were obtained by fusion of murine myeloma cells with spleen lymphocytes of either F344 rats or BALB/c mice immunized against the malignant F344 tracheal cell line, 2–10–1. Monoclonal antibodies were selected for their ability to bind to the immunizing cell line and not to normal tracheal epithelial cells. Results from quantitative binding assays indicated that such monoclonal antibodies recognized six epitopes. Each epitope was detected on four other malignant tracheal epithelial cell lines, and was also expressed during early preneoplastic cell passages (i.e. before the cells acquired the ability to produce carcinomas in vivo). Quantitative differences in epitope expression between non‐tumorigenic and tumorigenic passages of individual cell lines could not be detected for five of the epitope groups. However, two monoclonal antibodies, recognizing the same epitope, did show quantitative binding differences between non‐tumorigenic and tumorigenic cell passages on three of the five cell lines tested. Our results show that carcinogenaltered tracheal cell populations can be distinguished from non‐altered cells (by our current assay methods) by use of monoclonal antibodies. The suggest that the antigen expression is an early event associated with the transformation of rat tracheal epithelial cells in culture.

Original languageEnglish (US)
Pages (from-to)131-138
Number of pages8
JournalInternational Journal of Cancer
Volume33
Issue number1
DOIs
StatePublished - 1984
Externally publishedYes

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Epithelial Cells
Monoclonal Antibodies
Epitopes
Cell Line
Inbred F344 Rats
Hybridomas
In Vitro Techniques
Spleen
Cell Culture Techniques
Lymphocytes
Carcinoma
Antigens
Population

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Oncology
  • Cancer Research

Cite this

Monoclonal antibodies directed against rat tracheal epithelial cells transformed in vitro. / Braslawsky, Gary R.; Kennel, Stephen; Hand, R. E.; Nettesheim, Paul.

In: International Journal of Cancer, Vol. 33, No. 1, 1984, p. 131-138.

Research output: Contribution to journalArticle

Braslawsky, Gary R. ; Kennel, Stephen ; Hand, R. E. ; Nettesheim, Paul. / Monoclonal antibodies directed against rat tracheal epithelial cells transformed in vitro. In: International Journal of Cancer. 1984 ; Vol. 33, No. 1. pp. 131-138.
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