Monoclonal antibodies directed to different regions of vascular endothelial cadherin extracellular domain affect adhesion and clustering of the protein and modulate endothelial permeability

Monica Corada, Francesca-Fang Liao, Maria Lindgren, Maria Grazia Lampugnani, Ferruccio Breviario, Ronald Frank, William A. Muller, Daniel J. Hicklin, Peter Bohlen, Elisabetta Dejana

Research output: Contribution to journalArticle

222 Citations (Scopus)

Abstract

Vascular endothelial cadherin (VE-cadherin) is an endothelial cell-specific cadherin that plays an important role in the control of vascular organization. Blocking VE-cadherin antibodies strongly inhibit angiogenesis, and inactivation of VE-cadherin gene causes embryonic lethality due to a lack of correct organization and remodeling of the vasculature. Hence, inhibitors of VE-cadherin adhesive properties may constitute a tool to prevent tumor neovascularization. In this paper, we tested different monoclonal antibodies (mAbs) directed to human VE-cadherin ectodomain for their functional activity. Three mAbs (Cad 5, BV6, BV9) were able to increase paracellular permeability, inhibit VE-cadherin reorganization, and block angiogenesis in vitro. These mAbs could also induce endothelial cell apoptosis in vitro. Two additional mAbs, TEA 1.31 and Hec 1.2, had an intermediate or undetectable activity, respectively, in these assays. Epitope mapping studies show that BV6, BV9, TEA 1.31, and Hec 1.2 bound to a recombinant fragment spanning the extracellular juxtamembrane domains EC3 through EC4. In contrast, Cad 5 bound to the aminoterminal domain EC1. By peptide scanning analysis and competition experiments, we defined the sequences TIDLRY located on EC3 and KVFRVDAETGDVFAI on EC1 as the binding domain of BV6 and Cad 5, respectively. Overall, these results support the concept that VE-cadherin plays a relevant role on human endothelial cell properties. Antibodies directed to the extracellular domains EC1 but also EC3-EC4 affect VE-cadherin adhesion and clustering and alter endothelial cell permeability, apoptosis, and vascular structure formation.

Original languageEnglish (US)
Pages (from-to)1679-1684
Number of pages6
JournalBlood
Volume97
Issue number6
DOIs
StatePublished - Mar 15 2001

Fingerprint

Cluster Analysis
Permeability
Adhesion
Monoclonal Antibodies
Endothelial cells
Proteins
Endothelial Cells
Apoptosis
Epitope Mapping
cadherin 5
Antibodies
Cadherins
Adhesives
Blood Vessels
Tumors
Epitopes
Assays
Genes
Scanning
Peptides

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Monoclonal antibodies directed to different regions of vascular endothelial cadherin extracellular domain affect adhesion and clustering of the protein and modulate endothelial permeability. / Corada, Monica; Liao, Francesca-Fang; Lindgren, Maria; Lampugnani, Maria Grazia; Breviario, Ferruccio; Frank, Ronald; Muller, William A.; Hicklin, Daniel J.; Bohlen, Peter; Dejana, Elisabetta.

In: Blood, Vol. 97, No. 6, 15.03.2001, p. 1679-1684.

Research output: Contribution to journalArticle

Corada, Monica ; Liao, Francesca-Fang ; Lindgren, Maria ; Lampugnani, Maria Grazia ; Breviario, Ferruccio ; Frank, Ronald ; Muller, William A. ; Hicklin, Daniel J. ; Bohlen, Peter ; Dejana, Elisabetta. / Monoclonal antibodies directed to different regions of vascular endothelial cadherin extracellular domain affect adhesion and clustering of the protein and modulate endothelial permeability. In: Blood. 2001 ; Vol. 97, No. 6. pp. 1679-1684.
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AU - Breviario, Ferruccio

AU - Frank, Ronald

AU - Muller, William A.

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AU - Dejana, Elisabetta

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AB - Vascular endothelial cadherin (VE-cadherin) is an endothelial cell-specific cadherin that plays an important role in the control of vascular organization. Blocking VE-cadherin antibodies strongly inhibit angiogenesis, and inactivation of VE-cadherin gene causes embryonic lethality due to a lack of correct organization and remodeling of the vasculature. Hence, inhibitors of VE-cadherin adhesive properties may constitute a tool to prevent tumor neovascularization. In this paper, we tested different monoclonal antibodies (mAbs) directed to human VE-cadherin ectodomain for their functional activity. Three mAbs (Cad 5, BV6, BV9) were able to increase paracellular permeability, inhibit VE-cadherin reorganization, and block angiogenesis in vitro. These mAbs could also induce endothelial cell apoptosis in vitro. Two additional mAbs, TEA 1.31 and Hec 1.2, had an intermediate or undetectable activity, respectively, in these assays. Epitope mapping studies show that BV6, BV9, TEA 1.31, and Hec 1.2 bound to a recombinant fragment spanning the extracellular juxtamembrane domains EC3 through EC4. In contrast, Cad 5 bound to the aminoterminal domain EC1. By peptide scanning analysis and competition experiments, we defined the sequences TIDLRY located on EC3 and KVFRVDAETGDVFAI on EC1 as the binding domain of BV6 and Cad 5, respectively. Overall, these results support the concept that VE-cadherin plays a relevant role on human endothelial cell properties. Antibodies directed to the extracellular domains EC1 but also EC3-EC4 affect VE-cadherin adhesion and clustering and alter endothelial cell permeability, apoptosis, and vascular structure formation.

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