Monoclonal antibodies from NZW × BXSB F1 mice to β2-glycoprotein I and cardiolipin

Species specificity and charge-dependent binding

Marc Monestier, David A. Kandiah, Steven Kouts, Kristine E. Novick, Gaik Lin Ong, Marko Radic, Steven A. Krilis

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

NZW × BXSB F1 mice develop a systemic autoimmune syndrome with various lupus-like manifestations. Male animals develop a degenerative coronary disease with myocardial infarction, resulting in death before 6 mo of age. The presence in these mice of anti-phospholipid Abs reacting with β2-glycoprotein I may contribute to the pathogenesis of the cardiovascular lesions. β2-glycoprotein I, a plasma protein implicated in various aspects of the coagulation pathway, is also the target of autoantibodies in humans with the anti-phospholipid syndrome. We obtained several mAbs from NZW × BXSB F1 mice that were selected for binding to cardiolipin. Two mAbs are specific for β2-glycoprotein I and display a species-dependent pattern with preferential reactivity to mouse β2-glycoprotein I. The other mAbs display charge-mediated interactions with anionic phospholipids in the absence of β2-glycoprotein I. The analysis of the V region sequences of the mAbs suggests that cationic residues in the H chain complementarity-determining region 3 are important for their phospholipid reactivity. The structural features of the VH-D-JH junctions of these mAbs further support the view that an increased frequency of unusual V(D)J rearrangements directly contributes to the development of murine autoimmunity.

Original languageEnglish (US)
Pages (from-to)2631-2641
Number of pages11
JournalJournal of Immunology
Volume156
Issue number7
StatePublished - Apr 1 1996
Externally publishedYes

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Species Specificity
Cardiolipins
Glycoproteins
Monoclonal Antibodies
Phospholipids
Complementarity Determining Regions
Antiphospholipid Syndrome
Autoimmunity
Autoantibodies
Coronary Disease
Blood Proteins
Myocardial Infarction

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Monestier, M., Kandiah, D. A., Kouts, S., Novick, K. E., Ong, G. L., Radic, M., & Krilis, S. A. (1996). Monoclonal antibodies from NZW × BXSB F1 mice to β2-glycoprotein I and cardiolipin: Species specificity and charge-dependent binding. Journal of Immunology, 156(7), 2631-2641.

Monoclonal antibodies from NZW × BXSB F1 mice to β2-glycoprotein I and cardiolipin : Species specificity and charge-dependent binding. / Monestier, Marc; Kandiah, David A.; Kouts, Steven; Novick, Kristine E.; Ong, Gaik Lin; Radic, Marko; Krilis, Steven A.

In: Journal of Immunology, Vol. 156, No. 7, 01.04.1996, p. 2631-2641.

Research output: Contribution to journalArticle

Monestier, M, Kandiah, DA, Kouts, S, Novick, KE, Ong, GL, Radic, M & Krilis, SA 1996, 'Monoclonal antibodies from NZW × BXSB F1 mice to β2-glycoprotein I and cardiolipin: Species specificity and charge-dependent binding', Journal of Immunology, vol. 156, no. 7, pp. 2631-2641.
Monestier, Marc ; Kandiah, David A. ; Kouts, Steven ; Novick, Kristine E. ; Ong, Gaik Lin ; Radic, Marko ; Krilis, Steven A. / Monoclonal antibodies from NZW × BXSB F1 mice to β2-glycoprotein I and cardiolipin : Species specificity and charge-dependent binding. In: Journal of Immunology. 1996 ; Vol. 156, No. 7. pp. 2631-2641.
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