Monoclonal antibodies from rats immunized with fragment D of human fibrinogen

Stephen Kennel, James Chen, Patricia K. Lankford, Linda J. Foote

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Fischer rats were immunized with fragment D (Fg-D) of human fibrinogen (Fg) to obtain antibody specific for neoantigens unique to this molecule. Absorption of serum with whole Fg indicated that some of the antibody produced reacted preferentially with Fg-D. Hybridoma cultures were prepared by fusion of immune rat spleen cells with mouse myeloma P3-X63-Ag8. Monoclonal antibodies obtained from these cultures fell into two classes: (a) Those reacting equally well with Fg and Fg-D. (b) Those reacting preferentially but not absolutely with Fg-D. Antibody from hybridoma 104-14, a member of the first group had an affinity for Fg-D of 1.5 × 109 M-1 while antibodies from 106-59 and 106-71 (group 2) demonstrated much lower affinities of 1.0 × 107 and 4.7 × 106 M-1, respectively. The cross reactivity of antibodies in the second group indicated that they react with protein conformations that are altered during production of Fg-D from Fg.

Original languageEnglish (US)
Pages (from-to)309-320
Number of pages12
JournalThrombosis Research
Volume22
Issue number3
DOIs
StatePublished - May 1 1981
Externally publishedYes

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Fibrinogen
Monoclonal Antibodies
Antibodies
Hybridomas
Protein Conformation
Inbred F344 Rats
Spleen
fibrinogen D fragment
Serum

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine
  • Hematology

Cite this

Monoclonal antibodies from rats immunized with fragment D of human fibrinogen. / Kennel, Stephen; Chen, James; Lankford, Patricia K.; Foote, Linda J.

In: Thrombosis Research, Vol. 22, No. 3, 01.05.1981, p. 309-320.

Research output: Contribution to journalArticle

Kennel, Stephen ; Chen, James ; Lankford, Patricia K. ; Foote, Linda J. / Monoclonal antibodies from rats immunized with fragment D of human fibrinogen. In: Thrombosis Research. 1981 ; Vol. 22, No. 3. pp. 309-320.
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