Monoclonal antibodies to resistance-related forms of cytochrome P450 in Drosophila melanogaster

Scott S. Sundseth, Stephen Kennel, Larry C. Waters

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Drosophila cytochrome P450 is resolved by sodium dodecylsulfate-polyacrylamide gel electrophoresis into two subsets. P450-A4 4 P450-A is used to indicate all isozymic forms of P450 that electrophorese as protein band A, 59.3 kDa (see Ref. (15)), and that appear to be present in all Drosophila strains; P450-B indicates those that electrophorese as protein band B, 55.8 kDa, and whose expression is strain dependent., ∼59 kDa, is ubiquitous among strains whereas P450-B, ∼56 kDa, is expressed in strains resistant to insecticides. Monoclonal antibodies (MoAbs) to each of these subsets were produced. MoAbs 13-2e and 8-1d were specific for epitopes in the P450-A and P450-B subsets, respectively. Although P450-B is virtually undetectable by protein staining in insecticide-susceptible strains, immunoreactive P450-B was detected in these strains. However, immunoreactive P450-B was 10 to 20 times higher in strains selected for DDT resistance than in was shown to be 10 to 100 times higher than in the unselected controls. These and previous data show that P450-B expression is regulated by resistance genes on the second and third chromosomes of Drosophila and is correlated with resistance to phenylurea, DDT, and malathion, with N-nitrosodimethylamine demethylase activity and with immunoreactivity with MoAb 8-1d.

Original languageEnglish (US)
Pages (from-to)176-188
Number of pages13
JournalPesticide Biochemistry and Physiology
Volume33
Issue number2
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

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Drosophila melanogaster
cytochrome P-450
Cytochrome P-450 Enzyme System
Drosophila
monoclonal antibodies
DDT
Monoclonal Antibodies
Insecticides
Malathion
Cytochrome P-450 CYP2E1
DDT (pesticide)
Epitopes
Polyacrylamide Gel Electrophoresis
Proteins
Chromosomes
Sodium
Staining and Labeling
insecticides
N-nitrosodimethylamine
proteins

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Health, Toxicology and Mutagenesis

Cite this

Monoclonal antibodies to resistance-related forms of cytochrome P450 in Drosophila melanogaster. / Sundseth, Scott S.; Kennel, Stephen; Waters, Larry C.

In: Pesticide Biochemistry and Physiology, Vol. 33, No. 2, 01.01.1989, p. 176-188.

Research output: Contribution to journalArticle

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