Monoclonal antibodies to the hydrogenase of Thiocapsa roseopersicina

Gabor Tigyi, Kornél L. Kovács

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Monoclonal antibodies were produced to electrophoretically pure hydrogenase from Thiocapsa roseopersicina. Protein immunoelectroblotting was used to identify the hydrogenase-specific antibodies. Among the 18 monoclonal antibodies selected by enzyme immunoassay, three were found to react with highly immunogenic trace contaminating proteins. One cell line produced antibody that inhibitied hydrogenase activity. This was the first specific inhibitor of the hydrogenase function. The results suggest that monoclonal antibodies could provide valuable new informations about the enzyme structure as well.

Original languageEnglish (US)
Pages (from-to)329-333
Number of pages5
JournalCurrent Microbiology
Volume11
Issue number6
DOIs
StatePublished - Nov 1 1984
Externally publishedYes

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Thiocapsa roseopersicina
Hydrogenase
Monoclonal Antibodies
Antibodies
Immunoenzyme Techniques
Immunoblotting
Proteins
Cell Line
Enzymes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Monoclonal antibodies to the hydrogenase of Thiocapsa roseopersicina. / Tigyi, Gabor; Kovács, Kornél L.

In: Current Microbiology, Vol. 11, No. 6, 01.11.1984, p. 329-333.

Research output: Contribution to journalArticle

Tigyi, Gabor ; Kovács, Kornél L. / Monoclonal antibodies to the hydrogenase of Thiocapsa roseopersicina. In: Current Microbiology. 1984 ; Vol. 11, No. 6. pp. 329-333.
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