Monoclonal antibody against human sperm-specific YLP12 peptide sequence involved in oocyte binding

Rajesh K. Naz, Subhash Chauhan, R. N. Trivedi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The monoclonal antibody (mAb) against YLP12 peptide was raised and its immunobiological properties were examined. In the Western blot procedure, the YLP12 mAb recognized a specific protein band of ∼50 ± 5 kD in human sperm extract and ∼72 ± 5 kD in human testis extract. The myeloma Ig control did not recognize these specific protein bands. In the immunofluorescence studies, the YLP12 mAb, and not the myeloma Ig, predominantly reacted with the acrosome regions of methanol-fixed human sperm. In the acrosome reaction assay, the YLP12 mAb showed a significant (p < .001) and a concentration-dependent inhibition of acrosome reaction. The myeloma Ig did not affect the acrosome reaction. There was no apparent effect of antibodies on sperm motility. Thus, the monoclonal antibody, if humanized by genetic engineering technology, may provide a useful immunocontraceptive agent.

Original languageEnglish (US)
Pages (from-to)169-175
Number of pages7
JournalArchives of Andrology
Volume48
Issue number3
DOIs
StatePublished - May 7 2002
Externally publishedYes

Fingerprint

Acrosome Reaction
Oocytes
Spermatozoa
Monoclonal Antibodies
Peptides
Antibodies, Monoclonal, Humanized
Acrosome
Genetic Engineering
Sperm Motility
Fluorescent Antibody Technique
Methanol
Testis
Proteins
Western Blotting
Technology
Antibodies

All Science Journal Classification (ASJC) codes

  • Endocrinology

Cite this

Monoclonal antibody against human sperm-specific YLP12 peptide sequence involved in oocyte binding. / Naz, Rajesh K.; Chauhan, Subhash; Trivedi, R. N.

In: Archives of Andrology, Vol. 48, No. 3, 07.05.2002, p. 169-175.

Research output: Contribution to journalArticle

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