Monoclonal antibody to human renal glomeruli cross-reacts with streptococcal M protein

P. Goroncy-Bermes, James Dale, E. H. Beachey, W. Opferkuch

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Two murine monoclonal antibodies (immunoglobulin M) evoked against human kidney tissue were examined for cross-reactivity with group A streptococci. A glomerulus-specific antibody, PMII.40.H.2, cross-reacted in an enzyme-linked immunosorbent assay with purified pepsin-extracted M proteins from type 6 and 12 streptococci, but not type 1, 3, 5, 19, and 24 M proteins. The cross-reactive monoclonal antibody also opsonized type 6 and 12 streptococci, indicating that it was directed against protective M protein epitopes that were exposed on the surfaces of these organisms. A control antibody, which was tubule specific, did not cross-react with any of the purified M proteins, nor did it opsonize any of the serotypes of streptococci tested. Western immunoblot experiments identified the glomerular cross-reactive antigen as a 43-kilodalton protein. The results demonstrate that M protein of group A streptococci and glomerular antigens of the human kidney possess cross-reactive determinants.

Original languageEnglish (US)
Pages (from-to)2416-2419
Number of pages4
JournalInfection and Immunity
Volume55
Issue number10
StatePublished - 1987
Externally publishedYes

Fingerprint

Streptococcus
Monoclonal Antibodies
Kidney
Proteins
Antigens
Antibodies
Pepsin A
Immunoglobulin M
streptococcal M protein
Epitopes
Western Blotting
Enzyme-Linked Immunosorbent Assay

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Goroncy-Bermes, P., Dale, J., Beachey, E. H., & Opferkuch, W. (1987). Monoclonal antibody to human renal glomeruli cross-reacts with streptococcal M protein. Infection and Immunity, 55(10), 2416-2419.

Monoclonal antibody to human renal glomeruli cross-reacts with streptococcal M protein. / Goroncy-Bermes, P.; Dale, James; Beachey, E. H.; Opferkuch, W.

In: Infection and Immunity, Vol. 55, No. 10, 1987, p. 2416-2419.

Research output: Contribution to journalArticle

Goroncy-Bermes, P, Dale, J, Beachey, EH & Opferkuch, W 1987, 'Monoclonal antibody to human renal glomeruli cross-reacts with streptococcal M protein', Infection and Immunity, vol. 55, no. 10, pp. 2416-2419.
Goroncy-Bermes, P. ; Dale, James ; Beachey, E. H. ; Opferkuch, W. / Monoclonal antibody to human renal glomeruli cross-reacts with streptococcal M protein. In: Infection and Immunity. 1987 ; Vol. 55, No. 10. pp. 2416-2419.
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