Morphine promotes the growth of HIV-1 in human peripheral blood mononuclear cell cocultures

Phillip K. Peterson, Burt Sharp, Genya Gekker, P. S. Portoghese, Kim Sannerud, Henry H. Balfour

Research output: Contribution to journalArticle

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Abstract

Because morphine has been shown to alter the function of human T lymphocytes and monocytes, we postulated that morphine would promote the growth of HIV-1 in these cells. To test this hypothesis, a coculture assay was used consisting of phytohemagglutinin (PHA)-activated peripheral blood mononuclear cells (PBMC) from normal donors and PBMC which had been infected with a viral isolate from an asymptomatic patient, HIV-1AT. The growth of HIV-1AT, as reflected by the concentration of p24 antigen in coculture supernatants, was markedly increased in cocultures that contained morphine. A bell-shaped dose-response curve was observed with three- to fourfold increased growth at a morphine concentration of 10-12 M. Augmentation of HIV-1AT growth by morphine required an interaction with the PHA-activated donor PBMC. Furthermore, potentiation of HIV-1AT growth by morphine was stereospecific and was antagonized by naloxone and β-funaltrexamine indicating involvement of an opiate receptor mechanism. These findings provide an additional explanation of how opiates could act as a cofactor in the pathogenesis of HIV-1 in intravenous drug users.

Original languageEnglish (US)
Pages (from-to)869-873
Number of pages5
JournalAIDS
Volume4
Issue number9
StatePublished - Sep 1990

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Coculture Techniques
Morphine
HIV-1
Blood Cells
Growth
HIV
Phytohemagglutinins
Opiate Alkaloids
Tissue Donors
Opioid Receptors
Naloxone
Drug Users
Monocytes
T-Lymphocytes
Antigens

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Peterson, P. K., Sharp, B., Gekker, G., Portoghese, P. S., Sannerud, K., & Balfour, H. H. (1990). Morphine promotes the growth of HIV-1 in human peripheral blood mononuclear cell cocultures. AIDS, 4(9), 869-873.

Morphine promotes the growth of HIV-1 in human peripheral blood mononuclear cell cocultures. / Peterson, Phillip K.; Sharp, Burt; Gekker, Genya; Portoghese, P. S.; Sannerud, Kim; Balfour, Henry H.

In: AIDS, Vol. 4, No. 9, 09.1990, p. 869-873.

Research output: Contribution to journalArticle

Peterson, PK, Sharp, B, Gekker, G, Portoghese, PS, Sannerud, K & Balfour, HH 1990, 'Morphine promotes the growth of HIV-1 in human peripheral blood mononuclear cell cocultures', AIDS, vol. 4, no. 9, pp. 869-873.
Peterson PK, Sharp B, Gekker G, Portoghese PS, Sannerud K, Balfour HH. Morphine promotes the growth of HIV-1 in human peripheral blood mononuclear cell cocultures. AIDS. 1990 Sep;4(9):869-873.
Peterson, Phillip K. ; Sharp, Burt ; Gekker, Genya ; Portoghese, P. S. ; Sannerud, Kim ; Balfour, Henry H. / Morphine promotes the growth of HIV-1 in human peripheral blood mononuclear cell cocultures. In: AIDS. 1990 ; Vol. 4, No. 9. pp. 869-873.
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