Morphological and electrophysiological properties of atypically oriented layer 2 pyramidal cells of the juvenile rat neocortex

J. F.M. Van Brederode, Robert Foehring, W. J. Spain

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Abstract

We used whole-cell patch clamp recordings combined with intracellular dye-filling to examine the morphological and electrophysiological properties of atypically oriented pyramidal cells located at the layer 1/2 border of the juvenile rat neocortex. Orientation of the apical dendrite varied from oblique (>20°from vertical) to truly horizontal (90°from vertical). The length of the apical dendrite ranged from 150 to 400μm. The total horizontal domain of the dendritic tree (including basal dendrites) of the longest horizontal pyramids exceeded 500μm, but we also found short horizontal cells with horizontal dendritic domains of less than 300μm. In addition, atypically oriented pyramids had long horizontal axon collaterals in layer 1/2. Electrophysiologically, atypically oriented pyramidal cells had intrinsic membrane properties similar to regularly oriented pyramids that have been described in the superficial layers at this age in the rat. Cells that fired repetitively were all regular spiking. In addition, we identified a subgroup of neurons (20%) in this sample, which were unable to fire more than a few spikes at the beginning of the current pulse.We suggest that the unique orientation and size of their dendritic trees and the length and arrangement of their local axons collaterals make atypically oriented pyramids in layer 2 ideally suited to perform horizontal integration of synaptic inputs in the neocortex. Copyright (C) 2000 IBRO.

Original languageEnglish (US)
Pages (from-to)851-861
Number of pages11
JournalNeuroscience
Volume101
Issue number4
DOIs
StatePublished - Nov 30 2000

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Pyramidal Cells
Neocortex
Dendrites
Axons
Coloring Agents
Neurons
Membranes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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Morphological and electrophysiological properties of atypically oriented layer 2 pyramidal cells of the juvenile rat neocortex. / Van Brederode, J. F.M.; Foehring, Robert; Spain, W. J.

In: Neuroscience, Vol. 101, No. 4, 30.11.2000, p. 851-861.

Research output: Contribution to journalArticle

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