Motor, cytoarchitectural and biochemical assessment of pharmacological neuroprotection against CNS damage induced by neonatal exposure to ionizing radiation

L. R. Guelman, M. A. Zorrilla Zubilete, H. Ríos, C. G. Di Toro, Alejandro Dopico, L. M. Zieher

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Exposure of neonatal rats to a 5 Gy single dose of X-irradiation induces permanent abnormalities in cerebellar cortex cytoarchitecture and neurochemistry and motor function. This rodent model constitutes an useful tool to evaluate morphological, neurochemical and motor changes induced by ionizing radiation and the possible restorative effects of potential or clearly established neuroprotective drugs. After selection and administration of a neuroprotective agent to neonatally irradiated rats, quantitative evaluations of motor behavior (gait), cerebellar cortex cytoarchitecture and cerebellar monoamine levels are performed. Data are compared to those of both saline-injected, X-irradiated, and saline-injected, sham-irradiated controls. Evaluation of data from the different experimental groups is performed at postnatal days 30 and 90. After this postnatal interval, radiation-induced damage of cerebellar function in nonprotected rodents is considered to be permanent. The longitudinal evaluation of various parameters in the different experimental groups through a multidisciplinary approach, allows determination of the variables that are more sensitive to X-irradiation-induced damage and/or neuroprotective agent-induced restoration. Given the well-known correspondence in cerebellar developmental stages between rodents and humans, this model and related studies bring health-related implications, considering the accidental or therapeutic exposure of developing human beings to ionizing radiation.

Original languageEnglish (US)
Pages (from-to)203-210
Number of pages8
JournalBrain Research Protocols
Volume7
Issue number3
DOIs
StatePublished - Jul 10 2001

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Neuroprotective Agents
Ionizing Radiation
Rodentia
Cerebellar Cortex
Pharmacology
Neurochemistry
Gait
Radiation
Health
Neuroprotection
Therapeutics

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Motor, cytoarchitectural and biochemical assessment of pharmacological neuroprotection against CNS damage induced by neonatal exposure to ionizing radiation. / Guelman, L. R.; Zorrilla Zubilete, M. A.; Ríos, H.; Di Toro, C. G.; Dopico, Alejandro; Zieher, L. M.

In: Brain Research Protocols, Vol. 7, No. 3, 10.07.2001, p. 203-210.

Research output: Contribution to journalArticle

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