Mouse eosinophil-associated ribonucleases: A unique subfamily expressed during hematopoiesis

Stephania Cormier, Kirsten A. Larson, Shubing Yuan, Trella L. Mitchell, Kari Lindenberger, Patricia Carrigan, Nancy A. Lee, James J. Lee

Research output: Contribution to journalArticle

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Abstract

A unique family of ribonucleases was identified by exhaustive screening of genomic and cDNA libraries using a probe derived from a gene encoding a ribonuclease stored in the mouse eosinophil secondary granule. This family contains at least 13 genes, which encode ribonucleases, and two potential pseudogenes. The conserved sequence identity among these genes (∼70%), as well as the isolation/purification of these ribonucleases from eosinophil secondary granules, has led us to conclude that these genes form a unique clade in the mouse that we have identified as the Ear (Eosinophil-associated ribonuclease) gene family. Analyses of the nucleotide substitutions that have occurred among these ribonuclease genes reveal that duplication events within this family have been episodic, occurring within three unique periods during the past 18 × 106 years. Moreover, comparisons of nonsynonymous (Ka) vs. synonymous (Ks) rates of nucleotide substitution show that although these genes conserve residues necessary for RNase activity, selective evolutionary pressure(s) exist such that acquired amino acid changes appear to be advantageous. The selective advantage of these amino acid changes is currently unclear, but the occurrence of this phenomenon in both the mouse and the human highlights the importance of these changes for Ear and, therefore, eosinophil effector function(s).

Original languageEnglish (US)
Pages (from-to)352-361
Number of pages10
JournalMammalian Genome
Volume12
Issue number5
DOIs
StatePublished - May 22 2001

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Eosinophil Cationic Protein
Pancreatic Ribonuclease
Hematopoiesis
Ribonucleases
Eosinophils
Genes
Nucleotides
Amino Acids
Pseudogenes
Gene Duplication
Genomic Library
Conserved Sequence
Gene Library
Pressure

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Cormier, S., Larson, K. A., Yuan, S., Mitchell, T. L., Lindenberger, K., Carrigan, P., ... Lee, J. J. (2001). Mouse eosinophil-associated ribonucleases: A unique subfamily expressed during hematopoiesis. Mammalian Genome, 12(5), 352-361. https://doi.org/10.1007/s003350020007

Mouse eosinophil-associated ribonucleases : A unique subfamily expressed during hematopoiesis. / Cormier, Stephania; Larson, Kirsten A.; Yuan, Shubing; Mitchell, Trella L.; Lindenberger, Kari; Carrigan, Patricia; Lee, Nancy A.; Lee, James J.

In: Mammalian Genome, Vol. 12, No. 5, 22.05.2001, p. 352-361.

Research output: Contribution to journalArticle

Cormier, S, Larson, KA, Yuan, S, Mitchell, TL, Lindenberger, K, Carrigan, P, Lee, NA & Lee, JJ 2001, 'Mouse eosinophil-associated ribonucleases: A unique subfamily expressed during hematopoiesis', Mammalian Genome, vol. 12, no. 5, pp. 352-361. https://doi.org/10.1007/s003350020007
Cormier S, Larson KA, Yuan S, Mitchell TL, Lindenberger K, Carrigan P et al. Mouse eosinophil-associated ribonucleases: A unique subfamily expressed during hematopoiesis. Mammalian Genome. 2001 May 22;12(5):352-361. https://doi.org/10.1007/s003350020007
Cormier, Stephania ; Larson, Kirsten A. ; Yuan, Shubing ; Mitchell, Trella L. ; Lindenberger, Kari ; Carrigan, Patricia ; Lee, Nancy A. ; Lee, James J. / Mouse eosinophil-associated ribonucleases : A unique subfamily expressed during hematopoiesis. In: Mammalian Genome. 2001 ; Vol. 12, No. 5. pp. 352-361.
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