Mouse model for sublethal Leptospira interrogans infection

Luciana Richer, Hari Hara Potula, Rita Melo, Ana Vieira, Maria Gomes-Solecki

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Although Leptospira can infect a wide range of mammalian species, most studies have been conducted in golden Syrian hamsters, a species particularly sensitive to acute disease. Chronic disease has been well characterized in the rat, one of the natural reservoir hosts. Studies in another asymptomatic reservoir host, the mouse, have occasionally been done and have limited infection to mice younger than 6 weeks of age.Weanalyzed the outcome of sublethal infection of C3H/HeJ mice older than age 10 weeks with Leptospira interrogans serovar Copenhageni. Infection led to bloodstream dissemination of Leptospira, which was followed by urinary shedding, body weight loss, hypothermia, and colonization of the kidney by live spirochetes 2 weeks after infection. In addition, Leptospira dissemination triggered inflammation in the kidney but not in the liver or lung, as determined by increased levels of mRNA transcripts for the keratinocyte-derived chemokine, RANTES, macrophage inflammatory protein 2, tumor necrosis factor alpha, interleukin- 1β, inducible nitric oxide synthase, interleukin-6, and gamma interferon in kidney tissue. The acquired humoral response to Leptospira infection led to the production of IgG mainly of the IgG1 subtype. Flow cytometric analysis of splenocytes from infected mice revealed that cellular expansion was primarily due to an increase in the levels of CD4+ and double-negative T cells (not CD8+ cells) and that CD4+ T cells acquired a CD44high CD62Llow effector phenotype not accompanied by increases in memory T cells. A mouse model for sublethal Leptospira infection allows understanding of the bacterial and host factors that lead to immune evasion, which can result in acute or chronic disease or resistance to infection (protection).

Original languageEnglish (US)
Pages (from-to)4693-4700
Number of pages8
JournalInfection and Immunity
Volume83
Issue number12
DOIs
StatePublished - Jan 1 2015

Fingerprint

Leptospira interrogans
Leptospira
Infection
Acute Disease
T-Lymphocytes
Kidney
Chronic Disease
Immunoglobulin G
Chemokine CXCL2
Immune Evasion
Chemokine CCL5
Spirochaetales
Disease Resistance
Inbred C3H Mouse
Mesocricetus
Nitric Oxide Synthase Type II
Hypothermia
Interleukin-1
Interferon-gamma
Weight Loss

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Mouse model for sublethal Leptospira interrogans infection. / Richer, Luciana; Potula, Hari Hara; Melo, Rita; Vieira, Ana; Gomes-Solecki, Maria.

In: Infection and Immunity, Vol. 83, No. 12, 01.01.2015, p. 4693-4700.

Research output: Contribution to journalArticle

Richer, Luciana ; Potula, Hari Hara ; Melo, Rita ; Vieira, Ana ; Gomes-Solecki, Maria. / Mouse model for sublethal Leptospira interrogans infection. In: Infection and Immunity. 2015 ; Vol. 83, No. 12. pp. 4693-4700.
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