Msi1 confers resistance to TRAIL by activating ERK in liver cancer cells

Nianli Liu, Tianran Chen, Xiaohong Wang, Darong Yang, Binbin Xue, Haizhen Zhu

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

To investigate TRAIL resistance mechanisms in hepatocellular carcinoma (HCC), we isolated a stable TRAIL-resistant sub-population of the HCC cell line LH86, designated LH86-TR. Differential activation of AKT was not responsible for acquisition of TRAIL resistance. Cells with both congenital and acquired resistance to TRAIL exhibited increased Msi1 expression, which conferred TRAIL resistance by activating ERK. Forced expression of Msi1 decreased the sensitivity of HCC cells to TRAIL both in vitro and in vivo. Conversely, shRNA-mediated depletion of Msi1 enhanced TRAIL efficacy. SiRNA-mediated depletion of ERK overcame TRAIL resistance. Hence, we conclude that Msi1 is a mediator of TRAIL resistance in HCC cells.

Original languageEnglish (US)
Pages (from-to)897-903
Number of pages7
JournalFEBS Letters
Volume589
Issue number8
DOIs
StatePublished - Apr 2 2015

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Liver Neoplasms
Liver
Small Interfering RNA
Hepatocellular Carcinoma
Chemical activation
Cells
Cell Line
Population

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Msi1 confers resistance to TRAIL by activating ERK in liver cancer cells. / Liu, Nianli; Chen, Tianran; Wang, Xiaohong; Yang, Darong; Xue, Binbin; Zhu, Haizhen.

In: FEBS Letters, Vol. 589, No. 8, 02.04.2015, p. 897-903.

Research output: Contribution to journalArticle

Liu, Nianli ; Chen, Tianran ; Wang, Xiaohong ; Yang, Darong ; Xue, Binbin ; Zhu, Haizhen. / Msi1 confers resistance to TRAIL by activating ERK in liver cancer cells. In: FEBS Letters. 2015 ; Vol. 589, No. 8. pp. 897-903.
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