Multi-generational pharmacophore modeling for ligands to the cholane steroid-recognition site in the β1 modulatory subunit of the BKCa channel

Jacob E. McMillan, Anna Bukiya, Camisha L. Terrell, Shivaputra A. Patil, Duane Miller, Alejandro Dopico, Abby L. Parrill

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Large conductance, voltage- and Ca2+-gated K+ (BKCa) channels play a critical role in smooth muscle contractility and thus represent an emerging therapeutic target for drug development to treat vascular disease, gastrointestinal, bladder and uterine disorders. Several compounds are known to target the ubiquitously expressed BKCa channel-forming α subunit. In contrast, just a few are known to target the BKCa modulatory β1 subunit, which is highly expressed in smooth muscle and scarce in most other tissues. Lack of available high-resolution structural data makes structure-based pharmacophore modeling of β1 subunit-dependent BKCa channel activators a major challenge. Following recent discoveries of novel BKCa channel activators that act via β1 subunit recognition, we performed ligand-based pharmacophore modeling that led to the successful creation and fine-tuning of a pharmacophore over several generations. Initial models were developed using physiologically active cholane steroids (bile acids) as template. However, as more compounds that act on BKCa β1 have been discovered, our model has been refined to improve accuracy. Database searching with our best-performing model has uncovered several novel compounds as candidate BKCa β1 subunit ligands. Eight of the identified compounds were experimentally screened and two proved to be activators of recombinant BKCa β1 complexes. One of these activators, sobetirome, differs substantially in structure from any previously reported activator.

Original languageEnglish (US)
Pages (from-to)174-183
Number of pages10
JournalJournal of Molecular Graphics and Modelling
Volume54
DOIs
StatePublished - Jan 1 2014

Fingerprint

Cholanes
steroids
Steroids
Ligands
smooth muscle
ligands
Muscle
Bile Acids and Salts
data structures
bladder
Tuning
Tissue
emerging
drugs
templates
Acids
tuning
Electric potential
disorders
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Spectroscopy
  • Physical and Theoretical Chemistry
  • Computer Graphics and Computer-Aided Design
  • Materials Chemistry

Cite this

Multi-generational pharmacophore modeling for ligands to the cholane steroid-recognition site in the β1 modulatory subunit of the BKCa channel. / McMillan, Jacob E.; Bukiya, Anna; Terrell, Camisha L.; Patil, Shivaputra A.; Miller, Duane; Dopico, Alejandro; Parrill, Abby L.

In: Journal of Molecular Graphics and Modelling, Vol. 54, 01.01.2014, p. 174-183.

Research output: Contribution to journalArticle

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