Multiple positive and negative cis-acting elements mediate induced arginase (CAR1) gene expression in Saccharomyces cerevisiae

L. Kovari, R. Sumrada, I. Kovari, Terrance Cooper

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Expression of the arginase (CAR1) gene in Saccharomyces cerevisiae is induced by arginine or its analog homoarginine. Induction has been previously shown to require a negatively acting upstream repression sequence, which maintains expression of the gene at a low level in the absence of inducer. The objective of this work was to identify the cis-acting elements responsible for CAR1 transcriptional activation and response to inducer. We identified three upstream activation sequences (UASs) that suppport transcriptional activation in a heterologous expression vector. Two of these UAS elements function in the absence of inducer, whereas the third functions only when inducer is present. One of the inducer-dependent UAS elements exhibits significant homology to the Sp1 factor-binding sites identified in simian virus 40 and various mammalian genes.

Original languageEnglish (US)
Pages (from-to)5087-5097
Number of pages11
JournalMolecular and Cellular Biology
Volume10
Issue number10
DOIs
StatePublished - Jan 1 1990

Fingerprint

Arginase
Transcriptional Activation
Saccharomyces cerevisiae
Homoarginine
Gene Expression
Simian virus 40
Genes
Arginine
Binding Sites

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Multiple positive and negative cis-acting elements mediate induced arginase (CAR1) gene expression in Saccharomyces cerevisiae. / Kovari, L.; Sumrada, R.; Kovari, I.; Cooper, Terrance.

In: Molecular and Cellular Biology, Vol. 10, No. 10, 01.01.1990, p. 5087-5097.

Research output: Contribution to journalArticle

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