Muscarinic receptor-mediated prostacyclin and cGMP synthesis in cultured vascular cells

N. Jaiswal, R. K. Jaiswal, Kafait Malik

Research output: Contribution to journalArticle

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Abstract

The purpose of the present study was to determine the subtype of muscarinic receptor involved in the action of cholinergic stimuli of synthesis of prostacyclin, measured as immunoreactive 6-keto-prostaglandin F(1α) (6-keto-PGF(1α)), and cGMP in bovine aortic endothelial and rabbit vascular smooth muscle cells. Acetylcholine and arecaidine propargyl ester, a selective M2 agonist, produced a dose-dependent increase in 6-keto-PGF(1α) output and cGMP formation in confluent endothelial cells but not in confluent vascular smooth muscle cells. McN-A-343, a selective M1 agonist, failed to alter basal 6-keto-PGF(1α) or cGMP synthesis. Acetylcholine- and arecaidine propargyl ester-induced 6-keto-PGF(1α) synthesis and cGMP formation in endothelial cells were attenuated by atropine, AF-DX 116 (M2 antagonist), and hexahydro-siladifenidol (M3 antagonist) but not by pirenzepine (M1 antagonist). The cyclooxygenase inhibitor indomethacin abolished 6-keto-PGF(1α) synthesis but not the increase in cGMP formation elicited by the cholinergic stimuli. Our data suggest that the effect of cholinergic stimuli to enhance prostacyclin and cGMP synthesis is mediated via activation of M2 and M3 receptors located on endothelial cells and that the increase in cGMP production is independent of prostaglandins.

Original languageEnglish (US)
Pages (from-to)101-106
Number of pages6
JournalMolecular Pharmacology
Volume40
Issue number1
StatePublished - 1991

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Prostaglandins F
Muscarinic Receptors
Epoprostenol
Blood Vessels
Cultured Cells
Cholinergic Agents
Endothelial Cells
Vascular Smooth Muscle
Acetylcholine
Smooth Muscle Myocytes
(4-(m-Chlorophenylcarbamoyloxy)-2-butynyl)trimethylammonium Chloride
Pirenzepine
Cyclooxygenase Inhibitors
Atropine
Indomethacin
Prostaglandins
Rabbits

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Muscarinic receptor-mediated prostacyclin and cGMP synthesis in cultured vascular cells. / Jaiswal, N.; Jaiswal, R. K.; Malik, Kafait.

In: Molecular Pharmacology, Vol. 40, No. 1, 1991, p. 101-106.

Research output: Contribution to journalArticle

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