Muscarinic receptor subtypes are differentially distributed across brain stem respiratory nuclei

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Abstract

Cholinergic mechanisms are known to play a key role in the regulation of breathing, but the distribution of muscarinic receptor (mAChR) subtypes has not been localized within brain stem respiratory nuclei. This study examined the hypothesis that mAChR subtypes are heterogeneously distributed across brain stem nuclei that control breathing. With the use of in vitro receptor autoradiography, the results provide the first selective labeling and quantitative mapping of M1, M2, and M3 mAChR subtypes in cat brain stem regions known to regulate breathing. Among brain stem nuclei known to contain respiratory-related neurons, the greatest amount of mAChR binding was measured in the lateral and medial parabrachial nuclei and the lateral nucleus of the solitary tract. Fewer mAChRs were localized in nuclei comprising the ventral respiratory group (nucleus ambiguus, retrofacial nucleus) and ventral medulla (retrotrapezoid nucleus and ventrolateral medulla). The data provide an essential first step for future studies aiming to specify the regulatory role of mAChR subtypes within brain stem respiratory nuclei.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume268
Issue number6 12-6
StatePublished - 1995
Externally publishedYes

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Muscarinic Receptors
brain stem
Brain Stem
receptors
breathing
Respiration
Medulla Oblongata
Solitary Nucleus
cholinergic agents
autoradiography
Autoradiography
Cholinergic Agents
Cats
neurons
cats
Neurons
Parabrachial Nucleus

All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Physiology
  • Pulmonary and Respiratory Medicine
  • Agricultural and Biological Sciences(all)

Cite this

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abstract = "Cholinergic mechanisms are known to play a key role in the regulation of breathing, but the distribution of muscarinic receptor (mAChR) subtypes has not been localized within brain stem respiratory nuclei. This study examined the hypothesis that mAChR subtypes are heterogeneously distributed across brain stem nuclei that control breathing. With the use of in vitro receptor autoradiography, the results provide the first selective labeling and quantitative mapping of M1, M2, and M3 mAChR subtypes in cat brain stem regions known to regulate breathing. Among brain stem nuclei known to contain respiratory-related neurons, the greatest amount of mAChR binding was measured in the lateral and medial parabrachial nuclei and the lateral nucleus of the solitary tract. Fewer mAChRs were localized in nuclei comprising the ventral respiratory group (nucleus ambiguus, retrofacial nucleus) and ventral medulla (retrotrapezoid nucleus and ventrolateral medulla). The data provide an essential first step for future studies aiming to specify the regulatory role of mAChR subtypes within brain stem respiratory nuclei.",
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AU - Lydic, Ralph

AU - Baghdoyan, Helen

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N2 - Cholinergic mechanisms are known to play a key role in the regulation of breathing, but the distribution of muscarinic receptor (mAChR) subtypes has not been localized within brain stem respiratory nuclei. This study examined the hypothesis that mAChR subtypes are heterogeneously distributed across brain stem nuclei that control breathing. With the use of in vitro receptor autoradiography, the results provide the first selective labeling and quantitative mapping of M1, M2, and M3 mAChR subtypes in cat brain stem regions known to regulate breathing. Among brain stem nuclei known to contain respiratory-related neurons, the greatest amount of mAChR binding was measured in the lateral and medial parabrachial nuclei and the lateral nucleus of the solitary tract. Fewer mAChRs were localized in nuclei comprising the ventral respiratory group (nucleus ambiguus, retrofacial nucleus) and ventral medulla (retrotrapezoid nucleus and ventrolateral medulla). The data provide an essential first step for future studies aiming to specify the regulatory role of mAChR subtypes within brain stem respiratory nuclei.

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