Myosin isozyme distribution in rodent hindlimb skeletal muscle

Donald Thomason, K. M. Baldwin, R. E. Herrick

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The purpose of this study was to examine the distribution of myosin isozymes in rodent (Rattus novegicus) hindlimb skeletal muscles and regions of muscle known to have contrasting fiber-type composition. Muscle samples were analyzed for Ca2+-regulated myofibril adenosine triphosphate (ATPase) activity, Ca2+-activated myosin ATPase activity, myosin isozyme profile, and myosin light chain profile. Four isozymes of myosin were identified based on native protein and light chain electrophoresis patterns: one associated primarily with slow-twitch muscle (SM) and three associated primarily with fast-twitch muscle (FM). Multiple linear regression analysis of Ca2+-regulated myofibril ATPase activity (pCa 4) vs. measured isozyme profile was used to estimate the myofibril ATPase activities of the individual isozymes (FM1 = 0.86, FM2 = 0.52, FM3 = 0.31, and SM = 0.15 μmol P(i) formed·mg myofibril protein-1·min-1 at 25°C, n = 180, P < 0.001). Differences in the native isozyme profiles and myofibril ATPase activities between muscles and muscle regions of similar fiber type composition indicate that a given fiber type may not necessarily express a single isozyme profile. These data are consistent with the hypothesis that, among rodent hindlimb skeletal muscles and inherently their motor units, a range of myosin isozyme profiles exists that may provide a broad range of mechanical expression.

Original languageEnglish (US)
Pages (from-to)1923-1931
Number of pages9
JournalJournal of Applied Physiology
Volume60
Issue number6
StatePublished - 1986
Externally publishedYes

Fingerprint

Myosins
Hindlimb
Isoenzymes
Rodentia
Skeletal Muscle
Myofibrils
Muscles
Adenosine Triphosphatases
Myosin Light Chains
Calcium-Transporting ATPases
Electrophoresis
Linear Models
Proteins
Adenosine Triphosphate
Regression Analysis
Light

All Science Journal Classification (ASJC) codes

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Myosin isozyme distribution in rodent hindlimb skeletal muscle. / Thomason, Donald; Baldwin, K. M.; Herrick, R. E.

In: Journal of Applied Physiology, Vol. 60, No. 6, 1986, p. 1923-1931.

Research output: Contribution to journalArticle

Thomason, D, Baldwin, KM & Herrick, RE 1986, 'Myosin isozyme distribution in rodent hindlimb skeletal muscle', Journal of Applied Physiology, vol. 60, no. 6, pp. 1923-1931.
Thomason, Donald ; Baldwin, K. M. ; Herrick, R. E. / Myosin isozyme distribution in rodent hindlimb skeletal muscle. In: Journal of Applied Physiology. 1986 ; Vol. 60, No. 6. pp. 1923-1931.
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