N-terminal domain of borna disease virus G (p56) protein is sufficient for virus receptor recognition and cell entry

M. Perez, M. Watanabe, Michael Whitt, J. C. De la Torre

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Borna disease virus (BDV) surface glycoprotein (GP) (p56) has a predicted molecular mass of 56 kDa. Due to extensive posttranslational glycosylation the protein migrates as a polypeptide of 84 kDa (gp84). The processing of gp84 by the cellular protease furin generates gp43, which corresponds to the C-terminal part of gp84. Both gp84 and gp43 have been implicated in viral entry involving receptor-mediated endocytosis and pH-dependent fusion. We have investigated the domains of BDV p56 involved in virus entry. For this, we used a pseudotype approach based on a recently developed recombinant vesicular stomatitis virus (VSV) in which the gene for green fluorescent protein was substituted for the VSV G protein gene (VSVAG*). Complementation of VSVAG* with BDV p56 resulted in infectious VSVAG* pseudotypes that contained both BDV gp84 and gp43. BDV-VSV chimeric GPs that contained the N-terminal 244 amino acids of BDV p56 and amino acids 421 to 511 of VSV G protein were efficiently incorporated into VSVAG* particles, and the resulting pseudotype virions were neutralized by BDV-specific antiserum. These findings indicate that the N-terminal part of BDV p56 is sufficient for receptor recognition and virus entry.

Original languageEnglish (US)
Pages (from-to)7078-7085
Number of pages8
JournalJournal of Virology
Volume75
Issue number15
DOIs
StatePublished - Jul 24 2001

Fingerprint

Borna disease virus
Virus Receptors
Vesiculovirus
Proteins
proteins
cells
Virus Internalization
Vesicular Stomatitis
G-proteins
Furin
Viruses
Amino Acids
viruses
receptors
amino acids
virus receptors
Membrane Glycoproteins
endocytosis
glycosylation
Endocytosis

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

N-terminal domain of borna disease virus G (p56) protein is sufficient for virus receptor recognition and cell entry. / Perez, M.; Watanabe, M.; Whitt, Michael; De la Torre, J. C.

In: Journal of Virology, Vol. 75, No. 15, 24.07.2001, p. 7078-7085.

Research output: Contribution to journalArticle

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