Na+/H+ antiporter gene expression during monocytic differentiation of HL60 cells

Rao Gadiparthi, N. De Roux, C. Sardet, J. Pouyssegur, B. C. Berk

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

During differentiation of human promyelocytic HL60 cells into monocytes there are sustained increases in intracellular pH and Na+/H+ antiporter activity. Here we show that increased transcription and expression of the gene for the Na+/H+ antiporter precedes phorbol 12-myristate 13-acetate (PMA)-induced HL60 cell differentiation. PMA increased steady-state Na+/H+ antiporter mRNA levels ~50-fold within 8 h (at which time <15% of cells had differentiated). This increase was due to an increased transcriptional rate as determined by nuclear run on. Immunoprecipitation of [35S]methionine-labeled Na+/H+ antiporter using an antiporter fusion protein antibody (RP1-c28) showed an equivalent increase in Na+/H+ antiporter protein synthesis. The synthetic diacylglycerol, 1-oleolyl-2-acetylglycerol, an activator of protein kinase C, which unlike PMA did not cause differentiation, failed to induce Na+/H+ antiporter mRNA. Furthermore, inhibition of PMA-induced differentiation by either sphingosine or cycloheximide prevented accumulation of Na+/H+ antiporter mRNA. Together, these findings strongly suggest a close association of Na+/H+ antiporter induction with HL60 cell differentiation. The HL60 cell system is a promising model to study the mechanisms of Na+/H+ antiporter gene regulation and its function in differentiation.

Original languageEnglish (US)
Pages (from-to)13485-13488
Number of pages4
JournalJournal of Biological Chemistry
Volume266
Issue number21
StatePublished - Sep 9 1991

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Sodium-Hydrogen Antiporter
HL-60 Cells
Gene expression
Gene Expression
Acetates
Messenger RNA
Cell Differentiation
Antiporters
Sphingosine
Diglycerides
Transcription
Cycloheximide
Immunoprecipitation
Methionine
Protein Kinase C
Monocytes
Proteins
Fusion reactions
Genes
Association reactions

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Gadiparthi, R., De Roux, N., Sardet, C., Pouyssegur, J., & Berk, B. C. (1991). Na+/H+ antiporter gene expression during monocytic differentiation of HL60 cells. Journal of Biological Chemistry, 266(21), 13485-13488.

Na+/H+ antiporter gene expression during monocytic differentiation of HL60 cells. / Gadiparthi, Rao; De Roux, N.; Sardet, C.; Pouyssegur, J.; Berk, B. C.

In: Journal of Biological Chemistry, Vol. 266, No. 21, 09.09.1991, p. 13485-13488.

Research output: Contribution to journalArticle

Gadiparthi, R, De Roux, N, Sardet, C, Pouyssegur, J & Berk, BC 1991, 'Na+/H+ antiporter gene expression during monocytic differentiation of HL60 cells', Journal of Biological Chemistry, vol. 266, no. 21, pp. 13485-13488.
Gadiparthi, Rao ; De Roux, N. ; Sardet, C. ; Pouyssegur, J. ; Berk, B. C. / Na+/H+ antiporter gene expression during monocytic differentiation of HL60 cells. In: Journal of Biological Chemistry. 1991 ; Vol. 266, No. 21. pp. 13485-13488.
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