Na+/H+ antiporter gene expression increases during retinoic acid‐induced granulocytic differentiation of HL60 cells

Rao Gadiparthi, Claude Sardet, Jacques Pouysségur, Bradford C. Berk

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Abstract

During differentiation of human leukemic HL60 cells into granulocytes, sustained increases in intracellular pH and Na+/H+ antiporter activity have been observed. In the present study we report that retinoic acid (RA)‐induced granulocytic differentiation of HL60 cells causes an ∼18‐fold increase in the steady‐state mRNA levels for the Na+/H+ antiporter. This was due to an increase in the rate of Na+/H+ antiporter gene transcription as measured by nuclear run‐on analysis. Antiporter protein synthesis increased by seven‐fold during RA‐induced granulocytic differentiation of HL60 cells as measured by immunoprecipitation of 35S‐methionine‐labeled proteins with the RP1‐c28 Na+/H+ antiporter antibody. No increase in antiporter mRNA was observed in response to etretinate, an analogue of retinoic acid, which did not induce differentiation. Thus, Na+/H+ antiporter gene expression is associated with RA‐induced granulocytic differentiation of HL60 cells. The present findings and our previous data (Rao et al., 1991) demonstrate that Na+/H+ antiporter gene expression is a generalized feature of HL60 cell differentiation. © 1992 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)361-366
Number of pages6
JournalJournal of cellular physiology
Volume151
Issue number2
DOIs
StatePublished - Jan 1 1992

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Sodium-Hydrogen Antiporter
HL-60 Cells
Gene expression
Gene Expression
Antiporters
Tretinoin
Etretinate
Messenger RNA
Transcription
Immunoprecipitation
Granulocytes
Cell Differentiation
Proteins
Genes
Antibodies

All Science Journal Classification (ASJC) codes

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Na+/H+ antiporter gene expression increases during retinoic acid‐induced granulocytic differentiation of HL60 cells. / Gadiparthi, Rao; Sardet, Claude; Pouysségur, Jacques; Berk, Bradford C.

In: Journal of cellular physiology, Vol. 151, No. 2, 01.01.1992, p. 361-366.

Research output: Contribution to journalArticle

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AU - Berk, Bradford C.

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N2 - During differentiation of human leukemic HL60 cells into granulocytes, sustained increases in intracellular pH and Na+/H+ antiporter activity have been observed. In the present study we report that retinoic acid (RA)‐induced granulocytic differentiation of HL60 cells causes an ∼18‐fold increase in the steady‐state mRNA levels for the Na+/H+ antiporter. This was due to an increase in the rate of Na+/H+ antiporter gene transcription as measured by nuclear run‐on analysis. Antiporter protein synthesis increased by seven‐fold during RA‐induced granulocytic differentiation of HL60 cells as measured by immunoprecipitation of 35S‐methionine‐labeled proteins with the RP1‐c28 Na+/H+ antiporter antibody. No increase in antiporter mRNA was observed in response to etretinate, an analogue of retinoic acid, which did not induce differentiation. Thus, Na+/H+ antiporter gene expression is associated with RA‐induced granulocytic differentiation of HL60 cells. The present findings and our previous data (Rao et al., 1991) demonstrate that Na+/H+ antiporter gene expression is a generalized feature of HL60 cell differentiation. © 1992 Wiley‐Liss, Inc.

AB - During differentiation of human leukemic HL60 cells into granulocytes, sustained increases in intracellular pH and Na+/H+ antiporter activity have been observed. In the present study we report that retinoic acid (RA)‐induced granulocytic differentiation of HL60 cells causes an ∼18‐fold increase in the steady‐state mRNA levels for the Na+/H+ antiporter. This was due to an increase in the rate of Na+/H+ antiporter gene transcription as measured by nuclear run‐on analysis. Antiporter protein synthesis increased by seven‐fold during RA‐induced granulocytic differentiation of HL60 cells as measured by immunoprecipitation of 35S‐methionine‐labeled proteins with the RP1‐c28 Na+/H+ antiporter antibody. No increase in antiporter mRNA was observed in response to etretinate, an analogue of retinoic acid, which did not induce differentiation. Thus, Na+/H+ antiporter gene expression is associated with RA‐induced granulocytic differentiation of HL60 cells. The present findings and our previous data (Rao et al., 1991) demonstrate that Na+/H+ antiporter gene expression is a generalized feature of HL60 cell differentiation. © 1992 Wiley‐Liss, Inc.

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