Natural variation in neuron number in mice is linked to a major quantitative trait locus on Chr 11

Robert Williams, Richelle C. Strom, Dan Goldowitz

Research output: Contribution to journalArticle

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Abstract

Common genetic polymorphisms-as opposed to rare mutations-generate almost all heritable differences in the size and structure of the CNS. Surprisingly, these normal variants have not previously been mapped or cloned in any vertebrate species. In a recent paper (Williams et al., 1996a), we suggested that much of the variation in retinal ganglion cell number in mice, and the striking bimodality of strain averages, are caused by one or two quantitative trait loci (QTLs). To test this idea, and to map genes linked to this variable and highly heritable quantitative fait, we have counted ganglion cells in 38 recombinant inbred trains (BXD and BXH) derived from parental strains that have high and low cell numbers. A genome-wide search using simple and composite interval-mapping techniques revealed a major QTL on chromosome (Chr) 11 in a 3 cM interval between Hoxb and Krt1 (LOD = 6.8; genome-wide p = 0.001) and possible subsidiary QTLs on Chr 2 and Chr 8. The Chr 11 locus, neuron number control 1 (Nnc1), accounts for one third of the genetic variance among BXH strains and more than half of that among BXD strains, but Nnc1 has no known effects on brain weight, eye weight, or total retinal cell number. Three strong candidate genes have been mapped previously to the same region as Nnc1. These genes-Rara, Thra, and Erbb2- encode receptors for retinoic acid, thyroxine, and neuregulin, respectively. Each receptor is expressed in the retina during development, and their ligands affect the proliferation or survival of retinal cells.

Original languageEnglish (US)
Pages (from-to)138-146
Number of pages9
JournalJournal of Neuroscience
Volume18
Issue number1
StatePublished - 1998

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Chromosomes, Human, Pair 11
Quantitative Trait Loci
Cell Count
Neurons
Neuregulins
Genome
Genes
Weights and Measures
Chromosomes, Human, Pair 8
Retinoic Acid Receptors
Chromosomes, Human, Pair 2
Retinal Ganglion Cells
Genetic Polymorphisms
Thyroxine
Ganglia
Vertebrates
Retina
Cell Survival
Ligands
Mutation

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Natural variation in neuron number in mice is linked to a major quantitative trait locus on Chr 11. / Williams, Robert; Strom, Richelle C.; Goldowitz, Dan.

In: Journal of Neuroscience, Vol. 18, No. 1, 1998, p. 138-146.

Research output: Contribution to journalArticle

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abstract = "Common genetic polymorphisms-as opposed to rare mutations-generate almost all heritable differences in the size and structure of the CNS. Surprisingly, these normal variants have not previously been mapped or cloned in any vertebrate species. In a recent paper (Williams et al., 1996a), we suggested that much of the variation in retinal ganglion cell number in mice, and the striking bimodality of strain averages, are caused by one or two quantitative trait loci (QTLs). To test this idea, and to map genes linked to this variable and highly heritable quantitative fait, we have counted ganglion cells in 38 recombinant inbred trains (BXD and BXH) derived from parental strains that have high and low cell numbers. A genome-wide search using simple and composite interval-mapping techniques revealed a major QTL on chromosome (Chr) 11 in a 3 cM interval between Hoxb and Krt1 (LOD = 6.8; genome-wide p = 0.001) and possible subsidiary QTLs on Chr 2 and Chr 8. The Chr 11 locus, neuron number control 1 (Nnc1), accounts for one third of the genetic variance among BXH strains and more than half of that among BXD strains, but Nnc1 has no known effects on brain weight, eye weight, or total retinal cell number. Three strong candidate genes have been mapped previously to the same region as Nnc1. These genes-Rara, Thra, and Erbb2- encode receptors for retinoic acid, thyroxine, and neuregulin, respectively. Each receptor is expressed in the retina during development, and their ligands affect the proliferation or survival of retinal cells.",
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