Negative regulation of the expressions of cytokeratins 8 and 19 by SLUG repressor protein in human breast cells

Manish Tripathi, Smita Misra, Gautam Chaudhuri

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Invasiveness of tumor cells is often determined by the profile of their expressed genes. To determine the gene expression differences between an invasive and a non-invasive human breast tumor cells, we selected BT-549 (invasive) and MDA-MB-468 (non-invasive) cells, and compared their transcriptomes by cDNA microarray analysis. Among the significant differences in gene expressions, notable are the up-regulation of cytokeratins 8 and 19, and down-regulation of metallothioneins 1G and IL in MDA-MB-468 cells. Since MDA-MB-468 cells do not express SLUG, a member of a small family of E2-box-binding zinc finger silencer proteins, we studied whether the cytokeratin gene overexpressions in these cells are due to the absence of SLUG. Inducible expression of SLUG in MDA-MB-468 cells inhibited the expressions of the cytokeratin 8 and 19 but not others as was revealed by microarray analysis. Similarly, siRNA knock down of SLUG in BT-549 cells increased the expressions of those cytokeratin mRNAs. SLUG levels in the cell regulated the function of cytokeratins 8 and 19 gene promoters. We conclude that the expressions of cytokeratins and metallothioneins may be associated with the differential invasive behaviors of these breast tumor cells and SLUG may have regulatory roles in this process.

Original languageEnglish (US)
Pages (from-to)508-515
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume329
Issue number2
DOIs
StatePublished - Apr 8 2005
Externally publishedYes

Fingerprint

Keratin-8
Repressor Proteins
Keratin-19
Keratins
Tumors
Breast
Metallothionein
Genes
Cells
Microarrays
Gene expression
Small Interfering RNA
Zinc
Complementary DNA
Microarray Analysis
Messenger RNA
Breast Neoplasms
Proteins
Gene Expression
Zinc Fingers

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Negative regulation of the expressions of cytokeratins 8 and 19 by SLUG repressor protein in human breast cells. / Tripathi, Manish; Misra, Smita; Chaudhuri, Gautam.

In: Biochemical and Biophysical Research Communications, Vol. 329, No. 2, 08.04.2005, p. 508-515.

Research output: Contribution to journalArticle

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