Nerve growth factor combined with an epineural conduit for bridging a short nerve gap (10 mm). A study in rabbits

Antonia Barmpitsioti, Petros Konofaos, Ioannis Ignatiadis, Apostolos Papalois, Aristides B. Zoubos, Panagiotis N. Soucacos

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The purpose of this study was to evaluate the effect of direct administration of nerve growth factor (NGF) into an epineural conduit across a short nerve gap (10 mm) in a rabbit sciatic nerve model. The animals were divided into two groups. In group 1, n = 6, a 10-mm defect was created in the sciatic nerve and bridged with an epineural flap. A dose of 1 μg of NGF was locally administered daily for the first 21 days. NGF administration was made inside the epineural flap using a silicone reservoir connected to a silicone tube. In group 2, n = 6, the 10-mm defect was bridged with a nerve graft. This group did not receive any further treatment. At 13 weeks, all animals, before euthanasia, underwent electromyography (EMG) studies and then specimen sent for histology morphometric analysis. NGF administration ensured a significantly increased average number of myelinated axons per μm 2 (P = 0.028) and promoted fiber maturation (P = 0.031) and better EMG results (P = 0.046 for latency P = 0.048 for amplitude), compared with the control group. Although nerve grafts remain the gold standard for peripheral nerve repair, NGF-treated epineural conduits represent a good alternative, particularly when an unfavorable environment for nerve grafts is present.

Original languageEnglish (US)
Pages (from-to)545-550
Number of pages6
JournalMicrosurgery
Volume31
Issue number7
DOIs
StatePublished - Oct 1 2011
Externally publishedYes

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Nerve Growth Factor
Rabbits
Electromyography
Silicones
Sciatic Nerve
Transplants
Animal Euthanasia
Peripheral Nerves
Axons
Histology
Control Groups

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

Nerve growth factor combined with an epineural conduit for bridging a short nerve gap (10 mm). A study in rabbits. / Barmpitsioti, Antonia; Konofaos, Petros; Ignatiadis, Ioannis; Papalois, Apostolos; Zoubos, Aristides B.; Soucacos, Panagiotis N.

In: Microsurgery, Vol. 31, No. 7, 01.10.2011, p. 545-550.

Research output: Contribution to journalArticle

Barmpitsioti, Antonia ; Konofaos, Petros ; Ignatiadis, Ioannis ; Papalois, Apostolos ; Zoubos, Aristides B. ; Soucacos, Panagiotis N. / Nerve growth factor combined with an epineural conduit for bridging a short nerve gap (10 mm). A study in rabbits. In: Microsurgery. 2011 ; Vol. 31, No. 7. pp. 545-550.
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