Neural Crest Cells Contribute an Astrocyte-like Glial Population to the Spleen

Amanda J. Barlow-Anacker, Ming Fu, Christopher S. Erickson, Federica Bertocchini, Ankush Gosain

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Neural crest cells (NCC) are multi-potent cells of ectodermal origin that colonize diverse organs, including the gastrointestinal tract to form the enteric nervous system (ENS) and hematopoietic organs (bone marrow, thymus) where they participate in lymphocyte trafficking. Recent studies have implicated the spleen as an anatomic site for integration of inflammatory signals from the intestine with efferent neural inputs. We have previously observed alterations in splenic lymphocyte subsets in animals with defective migration of NCC that model Hirschsprung's disease, leading us to hypothesize that there may be a direct cellular contribution of NCC to the spleen. Here, we demonstrate that NCC colonize the spleen during embryogenesis and persist into adulthood. Splenic NCC display markers indicating a glial lineage and are arranged anatomically adjacent to blood vessels, pericytes and nerves, suggesting an astrocyte-like phenotype. Finally, we identify similar neural-crest derived cells in both the avian and non-human primate spleen, showing evolutionary conservation of these cells.

Original languageEnglish (US)
Article number45645
JournalScientific reports
Volume7
DOIs
StatePublished - Mar 28 2017

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Neural Crest
Neuroglia
Astrocytes
Spleen
Population
Enteric Nervous System
Hirschsprung Disease
Pericytes
Lymphocyte Subsets
Thymus Gland
Primates
Intestines
Embryonic Development
Blood Vessels
Gastrointestinal Tract
Bone Marrow
Lymphocytes
Phenotype

All Science Journal Classification (ASJC) codes

  • General

Cite this

Neural Crest Cells Contribute an Astrocyte-like Glial Population to the Spleen. / Barlow-Anacker, Amanda J.; Fu, Ming; Erickson, Christopher S.; Bertocchini, Federica; Gosain, Ankush.

In: Scientific reports, Vol. 7, 45645, 28.03.2017.

Research output: Contribution to journalArticle

Barlow-Anacker, Amanda J. ; Fu, Ming ; Erickson, Christopher S. ; Bertocchini, Federica ; Gosain, Ankush. / Neural Crest Cells Contribute an Astrocyte-like Glial Population to the Spleen. In: Scientific reports. 2017 ; Vol. 7.
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