Neurokinin-3 receptor distribution in rat and human brain: An immunohistochemical study

Darinka Mileusnic, J. M. Lee, D. J. Magnuson, M. J. Hejna, J. E. Krause, J. B. Lorens, S. A. Lorens

Research output: Contribution to journalArticle

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Abstract

Autoradiographic and immunohistochemical studies have shown that the neurokinin-3 receptor is widely distributed in the rodent CNS. Expression of the neurokinin-3 receptor in human brain, however, has been debated. These conflicting findings, as well as the poor resolution of autoradiographic images, prompted us to develop a polyclonal antibody against an oligopeptide derived from the carboxy-terminus consensus sequence of both the rat and human neurokinin-3 receptor ([C]ASTTSSFISSPYTSVDEYS, amino acids 434-452 of the rat neurokinin-3 receptor). Western blot analysis of both human and rat brain tissue revealed a major band in the molecular weight range 65,000- 67,000, the proposed molecular weight of the neurokinin-3 receptor based on its amino acid sequence and presumed glycosylation state. The distribution of selective high affinity neurokinin-3 receptor agonist [3H]senktide binding and neurokinin-3 receptor immunoreactivity were virtually identical in the brains of male Fischer 344 rats. The highest concentrations of neurokinin-3 receptors were observed in cortical layers IV-V; the basolateral amygdaloid nucleus; the hypothalamic paraventricular, perifornical and supraoptic nuclei; the zona incerta: and the entopeduncular and interpeduncular nuclei. [3H]senktide binding and neurokinin-3 receptor immunoreactivity were compared in homologous cortical areas of the human and rat brain. In contrast to the rat, autoradiographic analysis of normal control human brains (35-75 years) revealed a distinct and predominant superficial cortical labeling in the glia limitans and the cortical layer I. However, neurokinin-3 receptor immunoreactivity could be found not only in the superficial cortical layers, but also on pyramidal neurons and astrocytes in the neuropil and white matter. These findings suggest species differences in both the cellular and anatomical distribution of the neurokinin-3 receptor.

Original languageEnglish (US)
Pages (from-to)1269-1290
Number of pages22
JournalNeuroscience
Volume89
Issue number4
DOIs
StatePublished - Feb 17 1999
Externally publishedYes

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Neurokinin-3 Receptors
Brain
Entopeduncular Nucleus
Molecular Weight
Supraoptic Nucleus
Oligopeptides
Neuropil
Paraventricular Hypothalamic Nucleus
Pyramidal Cells
Inbred F344 Rats
Consensus Sequence
Amygdala
Glycosylation
Neuroglia
Astrocytes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Mileusnic, D., Lee, J. M., Magnuson, D. J., Hejna, M. J., Krause, J. E., Lorens, J. B., & Lorens, S. A. (1999). Neurokinin-3 receptor distribution in rat and human brain: An immunohistochemical study. Neuroscience, 89(4), 1269-1290. https://doi.org/10.1016/S0306-4522(98)00349-2

Neurokinin-3 receptor distribution in rat and human brain : An immunohistochemical study. / Mileusnic, Darinka; Lee, J. M.; Magnuson, D. J.; Hejna, M. J.; Krause, J. E.; Lorens, J. B.; Lorens, S. A.

In: Neuroscience, Vol. 89, No. 4, 17.02.1999, p. 1269-1290.

Research output: Contribution to journalArticle

Mileusnic, D, Lee, JM, Magnuson, DJ, Hejna, MJ, Krause, JE, Lorens, JB & Lorens, SA 1999, 'Neurokinin-3 receptor distribution in rat and human brain: An immunohistochemical study', Neuroscience, vol. 89, no. 4, pp. 1269-1290. https://doi.org/10.1016/S0306-4522(98)00349-2
Mileusnic, Darinka ; Lee, J. M. ; Magnuson, D. J. ; Hejna, M. J. ; Krause, J. E. ; Lorens, J. B. ; Lorens, S. A. / Neurokinin-3 receptor distribution in rat and human brain : An immunohistochemical study. In: Neuroscience. 1999 ; Vol. 89, No. 4. pp. 1269-1290.
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