Neuropeptide Y modulates the vascular response to periarterial nerve stimulation primarily by a postjunctional action in the isolated perfused rat kidney

W. F. Oellerich, Kafait Malik

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Abstract

Neuropeptide Y (NPY) is a 36-amino acid peptide that is colocalized and released with norepinephrine (NE) from central and peripheral adrenergic neurons and has been suggested to contribute to the control of vascular tone. This study was undertaken to assess the contribution of NPY at the vascular adrenergic neuroeffector junction in the rat kidney. Experiments were performed in isolated rat kidneys prelabeled with tritiated NE ([3H]NE). Infusion of NPY (1-50 nM) resulted in a dose-dependent increase in basal perfusion pressure and potentiated the vasoconstrictor response elicited by renal nerve stimulation (RNS; 0.5-4 Hz). NPY (10-50 nM) also potentiated the vasoconstrictor response elicited by exogenous NE (150 pmol). These effects of NPY were mimicked by [Leu31,Pro34]NPY, a NPY Y1 receptor agonist whereas [13-36]NPY, a NPY Y2 receptor agonist failed to alter the basal, RNS- or NE-induced increase in perfusion pressure. NPY (10 nM) and [Leu31,Pro34]NPY but not [13-36] NPY inhibited RNS-induced fractional tritium overflow only at high frequencies of stimulation (10 and 16 Hz) without altering basal tritium efflux. Periarterial nerve stimulation at 4 and 10 Hz resulted in release of immunoreactive NPY by 8- and 38-fold, respectively. These data indicate that NPY acts primarily at the postjunctional sites to produce renal vasoconstriction and to potentiate the vasoconstrictor response to RNS via Y1 receptors. Furthermore, NPY coreleased with adrenergic transmitter may also inhibit release of NE at higher frequencies of RNS (8-16 Hz) by acting on Y1 receptors at the prejunctional sites.

Original languageEnglish (US)
Pages (from-to)1321-1329
Number of pages9
JournalJournal of Pharmacology and Experimental Therapeutics
Volume266
Issue number3
StatePublished - 1993

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Neuropeptide Y
Blood Vessels
Kidney
Norepinephrine
Vasoconstrictor Agents
Tritium
Adrenergic Agents
Neuroeffector Junction
Perfusion
Neuropeptide Y Receptors
Pressure
Adrenergic Neurons
Vasoconstriction
Amino Acids

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

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abstract = "Neuropeptide Y (NPY) is a 36-amino acid peptide that is colocalized and released with norepinephrine (NE) from central and peripheral adrenergic neurons and has been suggested to contribute to the control of vascular tone. This study was undertaken to assess the contribution of NPY at the vascular adrenergic neuroeffector junction in the rat kidney. Experiments were performed in isolated rat kidneys prelabeled with tritiated NE ([3H]NE). Infusion of NPY (1-50 nM) resulted in a dose-dependent increase in basal perfusion pressure and potentiated the vasoconstrictor response elicited by renal nerve stimulation (RNS; 0.5-4 Hz). NPY (10-50 nM) also potentiated the vasoconstrictor response elicited by exogenous NE (150 pmol). These effects of NPY were mimicked by [Leu31,Pro34]NPY, a NPY Y1 receptor agonist whereas [13-36]NPY, a NPY Y2 receptor agonist failed to alter the basal, RNS- or NE-induced increase in perfusion pressure. NPY (10 nM) and [Leu31,Pro34]NPY but not [13-36] NPY inhibited RNS-induced fractional tritium overflow only at high frequencies of stimulation (10 and 16 Hz) without altering basal tritium efflux. Periarterial nerve stimulation at 4 and 10 Hz resulted in release of immunoreactive NPY by 8- and 38-fold, respectively. These data indicate that NPY acts primarily at the postjunctional sites to produce renal vasoconstriction and to potentiate the vasoconstrictor response to RNS via Y1 receptors. Furthermore, NPY coreleased with adrenergic transmitter may also inhibit release of NE at higher frequencies of RNS (8-16 Hz) by acting on Y1 receptors at the prejunctional sites.",
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AU - Oellerich, W. F.

AU - Malik, Kafait

PY - 1993

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N2 - Neuropeptide Y (NPY) is a 36-amino acid peptide that is colocalized and released with norepinephrine (NE) from central and peripheral adrenergic neurons and has been suggested to contribute to the control of vascular tone. This study was undertaken to assess the contribution of NPY at the vascular adrenergic neuroeffector junction in the rat kidney. Experiments were performed in isolated rat kidneys prelabeled with tritiated NE ([3H]NE). Infusion of NPY (1-50 nM) resulted in a dose-dependent increase in basal perfusion pressure and potentiated the vasoconstrictor response elicited by renal nerve stimulation (RNS; 0.5-4 Hz). NPY (10-50 nM) also potentiated the vasoconstrictor response elicited by exogenous NE (150 pmol). These effects of NPY were mimicked by [Leu31,Pro34]NPY, a NPY Y1 receptor agonist whereas [13-36]NPY, a NPY Y2 receptor agonist failed to alter the basal, RNS- or NE-induced increase in perfusion pressure. NPY (10 nM) and [Leu31,Pro34]NPY but not [13-36] NPY inhibited RNS-induced fractional tritium overflow only at high frequencies of stimulation (10 and 16 Hz) without altering basal tritium efflux. Periarterial nerve stimulation at 4 and 10 Hz resulted in release of immunoreactive NPY by 8- and 38-fold, respectively. These data indicate that NPY acts primarily at the postjunctional sites to produce renal vasoconstriction and to potentiate the vasoconstrictor response to RNS via Y1 receptors. Furthermore, NPY coreleased with adrenergic transmitter may also inhibit release of NE at higher frequencies of RNS (8-16 Hz) by acting on Y1 receptors at the prejunctional sites.

AB - Neuropeptide Y (NPY) is a 36-amino acid peptide that is colocalized and released with norepinephrine (NE) from central and peripheral adrenergic neurons and has been suggested to contribute to the control of vascular tone. This study was undertaken to assess the contribution of NPY at the vascular adrenergic neuroeffector junction in the rat kidney. Experiments were performed in isolated rat kidneys prelabeled with tritiated NE ([3H]NE). Infusion of NPY (1-50 nM) resulted in a dose-dependent increase in basal perfusion pressure and potentiated the vasoconstrictor response elicited by renal nerve stimulation (RNS; 0.5-4 Hz). NPY (10-50 nM) also potentiated the vasoconstrictor response elicited by exogenous NE (150 pmol). These effects of NPY were mimicked by [Leu31,Pro34]NPY, a NPY Y1 receptor agonist whereas [13-36]NPY, a NPY Y2 receptor agonist failed to alter the basal, RNS- or NE-induced increase in perfusion pressure. NPY (10 nM) and [Leu31,Pro34]NPY but not [13-36] NPY inhibited RNS-induced fractional tritium overflow only at high frequencies of stimulation (10 and 16 Hz) without altering basal tritium efflux. Periarterial nerve stimulation at 4 and 10 Hz resulted in release of immunoreactive NPY by 8- and 38-fold, respectively. These data indicate that NPY acts primarily at the postjunctional sites to produce renal vasoconstriction and to potentiate the vasoconstrictor response to RNS via Y1 receptors. Furthermore, NPY coreleased with adrenergic transmitter may also inhibit release of NE at higher frequencies of RNS (8-16 Hz) by acting on Y1 receptors at the prejunctional sites.

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