Neuroprotective effect of Acorus calamus against middle cerebral artery occlusion-induced ischaemia in rat

Pradeep Kumar Shukla, Vinay K. Khanna, M. Mohd Ali, Rakesh Maurya, M. Y. Khan, Rikhab C. Srimal

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Abstract

The neuroprotective potential of ethanol:water (1:1) extract of rhizomes of Acorus calamus (AC-002) has been investigated in middle cerebral artery occlusion (MCAO)-induced ischaemia in rats. A significant behavioural impairment in Rota-Rod performance and grid walking was observed in rats, 72 hours after MCAO as compared to sham-operated animals. These rats also exhibited an increase in lipid peroxidation (cortex - 157%, corpus striatum - 58%) and a decrease in glutathione levels (cortex - 59%, corpus striatum - 34%) and superoxide dismutase (SOD) activity (cortex - 64%, corpus striatum - 32%) as compared to sham-operated animals. Ischaemic rats treated with AC-002 (25 mg/kg, p.o.) exhibited a significant improvement in neurobehavioural performance viz. Rota-Rod performance and grid walking as compared to the MCAO group. Interestingly, treatment with AC-002 in MCAO rats significantly decreased malonaldialdehyde levels in cortex as compared to ischaemic rats. A significant increase in reduced glutathione levels and SOD activity was also observed both in cortex and corpus striatum in MCAO rats treated with AC-002 in comparison to MCAO rats. Treatment with AC-002 in MCAO rats also reduced the contralateral cortical infarct area (19%) as compared to MCAO rats (33%). Neurological function score was improved in the AC-002-treated rats as compared to the MCAO group. The results of the present study indicate the neuroprotective efficacy of A. calamus in the rat model of ischaemia.

Original languageEnglish (US)
Pages (from-to)187-194
Number of pages8
JournalHuman and Experimental Toxicology
Volume25
Issue number4
DOIs
StatePublished - Apr 1 2006

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Acorus
Middle Cerebral Artery Infarction
Neuroprotective Agents
Rats
Ischemia
Corpus Striatum
Superoxide Dismutase
Walking
Glutathione
Animals
Rhizome

All Science Journal Classification (ASJC) codes

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Neuroprotective effect of Acorus calamus against middle cerebral artery occlusion-induced ischaemia in rat. / Shukla, Pradeep Kumar; Khanna, Vinay K.; Ali, M. Mohd; Maurya, Rakesh; Khan, M. Y.; Srimal, Rikhab C.

In: Human and Experimental Toxicology, Vol. 25, No. 4, 01.04.2006, p. 187-194.

Research output: Contribution to journalArticle

Shukla, Pradeep Kumar ; Khanna, Vinay K. ; Ali, M. Mohd ; Maurya, Rakesh ; Khan, M. Y. ; Srimal, Rikhab C. / Neuroprotective effect of Acorus calamus against middle cerebral artery occlusion-induced ischaemia in rat. In: Human and Experimental Toxicology. 2006 ; Vol. 25, No. 4. pp. 187-194.
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