Neutralization by "antineoplastin" of insulin-activated nitric oxide synthase antibody and its effects in cancers

Asru K. Sinha, Krishnendu Acharya, Sujoy Bhattacharya, Sharmistha Chakraborty Patra, Manti Guha, Udayan Ray, Shyamali Basu Ray, Gausal A. Khan, Kushal Chakraborty, Jaydip Biswas, Sahana Mazumdar

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Purpose: The plasma level of nitric oxide (NO), that has been reported to possess various antineoplastic properties, was found to be diminished due to the impairment of insulin-activated nitric oxide synthase (IANOS) as a result of the appearance of a novel antibody (free light chain of IgG, Mr 44 kD) against the enzyme in the circulation in various cancers compared to normal control. Methods: We report here two NO-generating agents, antineoplastin I (a protein, Mr 5000) and antineoplastin II (an inorganic compound), which when applied to the skin of cancer patients were capable of neutralizing the antibody in vivo through the production of NO in the skin cells due to the stimulation of membrane IANOS of these cells and, subsequently, in erythrocytes in the circulation. Results: Neither antineoplastin I nor antineoplastin II itself enters into the circulation but due to the application of these agents on the skin, the NO synthesis in erythrocytes was normalized in these patients through "feedback" activation and amplification of IANOS activity by NO itself. Conclusion: It was found that the resumption of NO synthesis through the neutralization of antibody resulted in favorable modifications of various cancer-associated pathophysiologic consequences.

Original languageEnglish (US)
Pages (from-to)659-668
Number of pages10
JournalJournal of Cancer Research and Clinical Oncology
Volume128
Issue number12
DOIs
StatePublished - Dec 19 2002

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Nitric Oxide Synthase
Nitric Oxide
Insulin
Antibodies
Neoplasms
Erythrocytes
Skin
Skin Neoplasms
Neutralizing Antibodies
Antineoplastic Agents
Immunoglobulin G
Light
Membranes
Enzymes
Proteins
antineoplastin I

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Neutralization by "antineoplastin" of insulin-activated nitric oxide synthase antibody and its effects in cancers. / Sinha, Asru K.; Acharya, Krishnendu; Bhattacharya, Sujoy; Patra, Sharmistha Chakraborty; Guha, Manti; Ray, Udayan; Ray, Shyamali Basu; Khan, Gausal A.; Chakraborty, Kushal; Biswas, Jaydip; Mazumdar, Sahana.

In: Journal of Cancer Research and Clinical Oncology, Vol. 128, No. 12, 19.12.2002, p. 659-668.

Research output: Contribution to journalArticle

Sinha, AK, Acharya, K, Bhattacharya, S, Patra, SC, Guha, M, Ray, U, Ray, SB, Khan, GA, Chakraborty, K, Biswas, J & Mazumdar, S 2002, 'Neutralization by "antineoplastin" of insulin-activated nitric oxide synthase antibody and its effects in cancers', Journal of Cancer Research and Clinical Oncology, vol. 128, no. 12, pp. 659-668. https://doi.org/10.1007/s00432-002-0376-8
Sinha, Asru K. ; Acharya, Krishnendu ; Bhattacharya, Sujoy ; Patra, Sharmistha Chakraborty ; Guha, Manti ; Ray, Udayan ; Ray, Shyamali Basu ; Khan, Gausal A. ; Chakraborty, Kushal ; Biswas, Jaydip ; Mazumdar, Sahana. / Neutralization by "antineoplastin" of insulin-activated nitric oxide synthase antibody and its effects in cancers. In: Journal of Cancer Research and Clinical Oncology. 2002 ; Vol. 128, No. 12. pp. 659-668.
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