New aspects of sphingosine 1-phosphate signaling in mammalian cells

Nigel J. Pyne, Jaclyn S. Long, Sue Lee, Carolyn Loveridge, Laura Gillies, Susan Pyne

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Sphingosine 1-phosphate (S1P) is a bioactive lipid phosphate that binds to cell surface G-protein-coupled receptors (GPCR), but also can elicit intracellular actions. The role of S1P in cancer has been an area of significant interest and we have focused our research on two aspects that are of importance with respect to cancer. First, we have investigated how cross talk between S1P and growth factors might affect the pathophysiology of cancer cells. In this regard, we have demonstrated that S1P receptors function to re-programme the spatial signaling specificity of receptor tyrosine kinases and vice versa to modulate cell responses. Second, we have investigated spatial/temporal aspects of intracellular S1P signaling and how this might be de-regulated in cancer. This has involved studies on: (i) the interaction of sphingosine kinase 1 (which catalyses the phosphorylation of sphingosine to produce S1P) and phospholipase D in the Golgi apparatus linked to regulation of cell survival and (ii) the novel regulatory interaction between sphingosine kinase 1 and 2 and centrosomal S1P5 receptor linked to the regulation of mitosis in mammalian cells including MDA-MB-231 breast cancer cells. Therefore, we have focused on novel aspects of spatial and temporal S1P signaling that might enable this bioactive lipid phosphate to exhibit normal and aberrant function in health and disease respectively.

Original languageEnglish (US)
Pages (from-to)214-221
Number of pages8
JournalAdvances in Enzyme Regulation
Volume49
Issue number1
DOIs
StatePublished - Jun 10 2009
Externally publishedYes

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Lysosphingolipid Receptors
Neoplasms
Phosphates
Lipids
Phospholipase D
Sphingosine
Receptor Protein-Tyrosine Kinases
Golgi Apparatus
G-Protein-Coupled Receptors
Mitosis
sphingosine 1-phosphate
Cell Survival
Intercellular Signaling Peptides and Proteins
Membrane Proteins
Phosphorylation
Breast Neoplasms
Health
Research
sphingosine kinase

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

New aspects of sphingosine 1-phosphate signaling in mammalian cells. / Pyne, Nigel J.; Long, Jaclyn S.; Lee, Sue; Loveridge, Carolyn; Gillies, Laura; Pyne, Susan.

In: Advances in Enzyme Regulation, Vol. 49, No. 1, 10.06.2009, p. 214-221.

Research output: Contribution to journalArticle

Pyne, Nigel J. ; Long, Jaclyn S. ; Lee, Sue ; Loveridge, Carolyn ; Gillies, Laura ; Pyne, Susan. / New aspects of sphingosine 1-phosphate signaling in mammalian cells. In: Advances in Enzyme Regulation. 2009 ; Vol. 49, No. 1. pp. 214-221.
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