New bone formation around implants inserted on autologous and xenografts irradiated or not with IR laser light: A histomorphometric study in rabbits

Luiz Guilherme P. Soares, Edival Barreto de Magalhães Júnior, Cesar Augusto B. Magalhães, Cimara Ferreira, Aparecida Maria C. Marques, Antônio Luiz B. Pinheiro

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Use of biomaterials and light on bone grafts has been widely reported. This work assessed the influence of low-level laser therapy (LLLT) on bone volume (BV) and bone implant contact (BIC) interface around implants inserted in blocks of bovine or autologous bone grafts (autografts), irradiated or not, in rabbit femurs. Twenty-four adult rabbits were divided in 8 groups: AG: autograft; XG: xenograft; AG/L: autograft + laser; XG/L: xenograft + laser; AG/I: autograft + titanium (Ti) implant; XG/I: xenograft + Ti implant; AG/I/L: autograft + Ti implant + laser; and XG/I/L: xenograft + Ti implant + laser. The animals received the Ti implant after incorporation of the grafts. The laser parameters in the groups AG/L and XG/L were λ=780 nm, 70 mW, CW, 21.5 J/cm2, while in the groups AG/I/L and XG/I/L the following parameters were used: λ=780 nm, 70 mW, 0.5 cm2 (spot), 4 J/cm2 per point (4), 16 J/cm2 per session, 48 h interval x 12 sessions, CW, contact mode. LLLT was repeated every other day during 2 weeks. To avoid systemic effect, only one limb of each rabbit was double grafted. All animals were sacrificed 9 weeks after implantation. Specimens were routinely stained and histomorphometry carried out. Comparison of non-irradiated and irradiated grafts (AG/L versus AG and XG/L versus XG) showed that irradiation increased significantly BV on both grafts (p=0.05, p=0.001). Comparison between irradiated and non-irradiated grafts (AG/I/L versus AG/I and XG/I/L versus XG/I) showed a significant (p=0.02) increase of the BIC in autografts. The same was seen when xenografts were used, without significant difference. The results of this investigation suggest that the use of LLLT is effective for enhancing new bone formation with consequent increase of bone-implant interface in both autologous grafts and xenografts.

Original languageEnglish (US)
Pages (from-to)218-223
Number of pages6
JournalBrazilian dental journal
Volume24
Issue number3
DOIs
StatePublished - Aug 20 2013

Fingerprint

Heterografts
Osteogenesis
Lasers
Rabbits
Transplants
Light
Bone and Bones
Titanium
Biocompatible Materials
Femur
Extremities

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

New bone formation around implants inserted on autologous and xenografts irradiated or not with IR laser light : A histomorphometric study in rabbits. / Soares, Luiz Guilherme P.; de Magalhães Júnior, Edival Barreto; Magalhães, Cesar Augusto B.; Ferreira, Cimara; Marques, Aparecida Maria C.; Pinheiro, Antônio Luiz B.

In: Brazilian dental journal, Vol. 24, No. 3, 20.08.2013, p. 218-223.

Research output: Contribution to journalArticle

Soares, Luiz Guilherme P. ; de Magalhães Júnior, Edival Barreto ; Magalhães, Cesar Augusto B. ; Ferreira, Cimara ; Marques, Aparecida Maria C. ; Pinheiro, Antônio Luiz B. / New bone formation around implants inserted on autologous and xenografts irradiated or not with IR laser light : A histomorphometric study in rabbits. In: Brazilian dental journal. 2013 ; Vol. 24, No. 3. pp. 218-223.
@article{1d42c2c03ef74e3d90c8fcbc6d884fd3,
title = "New bone formation around implants inserted on autologous and xenografts irradiated or not with IR laser light: A histomorphometric study in rabbits",
abstract = "Use of biomaterials and light on bone grafts has been widely reported. This work assessed the influence of low-level laser therapy (LLLT) on bone volume (BV) and bone implant contact (BIC) interface around implants inserted in blocks of bovine or autologous bone grafts (autografts), irradiated or not, in rabbit femurs. Twenty-four adult rabbits were divided in 8 groups: AG: autograft; XG: xenograft; AG/L: autograft + laser; XG/L: xenograft + laser; AG/I: autograft + titanium (Ti) implant; XG/I: xenograft + Ti implant; AG/I/L: autograft + Ti implant + laser; and XG/I/L: xenograft + Ti implant + laser. The animals received the Ti implant after incorporation of the grafts. The laser parameters in the groups AG/L and XG/L were λ=780 nm, 70 mW, CW, 21.5 J/cm2, while in the groups AG/I/L and XG/I/L the following parameters were used: λ=780 nm, 70 mW, 0.5 cm2 (spot), 4 J/cm2 per point (4), 16 J/cm2 per session, 48 h interval x 12 sessions, CW, contact mode. LLLT was repeated every other day during 2 weeks. To avoid systemic effect, only one limb of each rabbit was double grafted. All animals were sacrificed 9 weeks after implantation. Specimens were routinely stained and histomorphometry carried out. Comparison of non-irradiated and irradiated grafts (AG/L versus AG and XG/L versus XG) showed that irradiation increased significantly BV on both grafts (p=0.05, p=0.001). Comparison between irradiated and non-irradiated grafts (AG/I/L versus AG/I and XG/I/L versus XG/I) showed a significant (p=0.02) increase of the BIC in autografts. The same was seen when xenografts were used, without significant difference. The results of this investigation suggest that the use of LLLT is effective for enhancing new bone formation with consequent increase of bone-implant interface in both autologous grafts and xenografts.",
author = "Soares, {Luiz Guilherme P.} and {de Magalh{\~a}es J{\'u}nior}, {Edival Barreto} and Magalh{\~a}es, {Cesar Augusto B.} and Cimara Ferreira and Marques, {Aparecida Maria C.} and Pinheiro, {Ant{\^o}nio Luiz B.}",
year = "2013",
month = "8",
day = "20",
doi = "10.1590/0103-6440201302186",
language = "English (US)",
volume = "24",
pages = "218--223",
journal = "Brazilian Dental Journal",
issn = "0103-6440",
publisher = "Associacao Brasileira de Divulgacao Cientifica",
number = "3",

}

TY - JOUR

T1 - New bone formation around implants inserted on autologous and xenografts irradiated or not with IR laser light

T2 - A histomorphometric study in rabbits

AU - Soares, Luiz Guilherme P.

AU - de Magalhães Júnior, Edival Barreto

AU - Magalhães, Cesar Augusto B.

AU - Ferreira, Cimara

AU - Marques, Aparecida Maria C.

AU - Pinheiro, Antônio Luiz B.

PY - 2013/8/20

Y1 - 2013/8/20

N2 - Use of biomaterials and light on bone grafts has been widely reported. This work assessed the influence of low-level laser therapy (LLLT) on bone volume (BV) and bone implant contact (BIC) interface around implants inserted in blocks of bovine or autologous bone grafts (autografts), irradiated or not, in rabbit femurs. Twenty-four adult rabbits were divided in 8 groups: AG: autograft; XG: xenograft; AG/L: autograft + laser; XG/L: xenograft + laser; AG/I: autograft + titanium (Ti) implant; XG/I: xenograft + Ti implant; AG/I/L: autograft + Ti implant + laser; and XG/I/L: xenograft + Ti implant + laser. The animals received the Ti implant after incorporation of the grafts. The laser parameters in the groups AG/L and XG/L were λ=780 nm, 70 mW, CW, 21.5 J/cm2, while in the groups AG/I/L and XG/I/L the following parameters were used: λ=780 nm, 70 mW, 0.5 cm2 (spot), 4 J/cm2 per point (4), 16 J/cm2 per session, 48 h interval x 12 sessions, CW, contact mode. LLLT was repeated every other day during 2 weeks. To avoid systemic effect, only one limb of each rabbit was double grafted. All animals were sacrificed 9 weeks after implantation. Specimens were routinely stained and histomorphometry carried out. Comparison of non-irradiated and irradiated grafts (AG/L versus AG and XG/L versus XG) showed that irradiation increased significantly BV on both grafts (p=0.05, p=0.001). Comparison between irradiated and non-irradiated grafts (AG/I/L versus AG/I and XG/I/L versus XG/I) showed a significant (p=0.02) increase of the BIC in autografts. The same was seen when xenografts were used, without significant difference. The results of this investigation suggest that the use of LLLT is effective for enhancing new bone formation with consequent increase of bone-implant interface in both autologous grafts and xenografts.

AB - Use of biomaterials and light on bone grafts has been widely reported. This work assessed the influence of low-level laser therapy (LLLT) on bone volume (BV) and bone implant contact (BIC) interface around implants inserted in blocks of bovine or autologous bone grafts (autografts), irradiated or not, in rabbit femurs. Twenty-four adult rabbits were divided in 8 groups: AG: autograft; XG: xenograft; AG/L: autograft + laser; XG/L: xenograft + laser; AG/I: autograft + titanium (Ti) implant; XG/I: xenograft + Ti implant; AG/I/L: autograft + Ti implant + laser; and XG/I/L: xenograft + Ti implant + laser. The animals received the Ti implant after incorporation of the grafts. The laser parameters in the groups AG/L and XG/L were λ=780 nm, 70 mW, CW, 21.5 J/cm2, while in the groups AG/I/L and XG/I/L the following parameters were used: λ=780 nm, 70 mW, 0.5 cm2 (spot), 4 J/cm2 per point (4), 16 J/cm2 per session, 48 h interval x 12 sessions, CW, contact mode. LLLT was repeated every other day during 2 weeks. To avoid systemic effect, only one limb of each rabbit was double grafted. All animals were sacrificed 9 weeks after implantation. Specimens were routinely stained and histomorphometry carried out. Comparison of non-irradiated and irradiated grafts (AG/L versus AG and XG/L versus XG) showed that irradiation increased significantly BV on both grafts (p=0.05, p=0.001). Comparison between irradiated and non-irradiated grafts (AG/I/L versus AG/I and XG/I/L versus XG/I) showed a significant (p=0.02) increase of the BIC in autografts. The same was seen when xenografts were used, without significant difference. The results of this investigation suggest that the use of LLLT is effective for enhancing new bone formation with consequent increase of bone-implant interface in both autologous grafts and xenografts.

UR - http://www.scopus.com/inward/record.url?scp=84881510732&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84881510732&partnerID=8YFLogxK

U2 - 10.1590/0103-6440201302186

DO - 10.1590/0103-6440201302186

M3 - Article

C2 - 23969909

AN - SCOPUS:84881510732

VL - 24

SP - 218

EP - 223

JO - Brazilian Dental Journal

JF - Brazilian Dental Journal

SN - 0103-6440

IS - 3

ER -