NF-κB-dependent MicroRNA-125b up-regulation promotes cell survival by targeting p38α upon ultraviolet radiation

Guangyun Tan, Jixiao Niu, Yuling Shi, Hongsheng Ouyang, Zhaohui Wu

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

UV-induced stress response involves expression change of a myriad of genes, which play critical roles in modulating cell cycle arrest, DNA repair, and cell survival. Alteration of micro-RNAs has been found in cells exposed to UV, yet their function in UV stress response remains elusive. Here, we show that UV radiation induces up-regulation of miR-125b, which negatively regulates p38α expression through targeting its 3′-UTR. Increase of miR-125b depends on UV-induced NF-κB activation, which enhances miR-125b gene transcription upon UV radiation. The DNA damage-responsive kinase ATM(ataxia telangiectasia mutated) is indispensable for UV-induced NF-κB activation, which may regulate p38α activation and IKKβ-dependent IκBα degradation in response to UV. Consequently, repression of p38α by miR-125b prohibits prolonged hyperactivation of p38α by UV radiation, which is required for protecting cells from UV-induced apoptosis. Altogether, our data support a critical role of NF-κB-dependent up-regulation of miR-125b, which forms a negative feedback loop to repress p38α activation and promote cell survival upon UV radiation.

Original languageEnglish (US)
Pages (from-to)33036-33047
Number of pages12
JournalJournal of Biological Chemistry
Volume287
Issue number39
DOIs
StatePublished - Sep 21 2012

Fingerprint

MicroRNAs
Ultraviolet radiation
Cell Survival
Up-Regulation
Chemical activation
Cells
Radiation
Genes
Ataxia Telangiectasia
DNA
3' Untranslated Regions
Transcription
Cell Cycle Checkpoints
DNA Repair
DNA Damage
Repair
Phosphotransferases
Apoptosis
Feedback
Degradation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

NF-κB-dependent MicroRNA-125b up-regulation promotes cell survival by targeting p38α upon ultraviolet radiation. / Tan, Guangyun; Niu, Jixiao; Shi, Yuling; Ouyang, Hongsheng; Wu, Zhaohui.

In: Journal of Biological Chemistry, Vol. 287, No. 39, 21.09.2012, p. 33036-33047.

Research output: Contribution to journalArticle

Tan, Guangyun ; Niu, Jixiao ; Shi, Yuling ; Ouyang, Hongsheng ; Wu, Zhaohui. / NF-κB-dependent MicroRNA-125b up-regulation promotes cell survival by targeting p38α upon ultraviolet radiation. In: Journal of Biological Chemistry. 2012 ; Vol. 287, No. 39. pp. 33036-33047.
@article{d757e1faa80a48798a9251d6428c6a41,
title = "NF-κB-dependent MicroRNA-125b up-regulation promotes cell survival by targeting p38α upon ultraviolet radiation",
abstract = "UV-induced stress response involves expression change of a myriad of genes, which play critical roles in modulating cell cycle arrest, DNA repair, and cell survival. Alteration of micro-RNAs has been found in cells exposed to UV, yet their function in UV stress response remains elusive. Here, we show that UV radiation induces up-regulation of miR-125b, which negatively regulates p38α expression through targeting its 3′-UTR. Increase of miR-125b depends on UV-induced NF-κB activation, which enhances miR-125b gene transcription upon UV radiation. The DNA damage-responsive kinase ATM(ataxia telangiectasia mutated) is indispensable for UV-induced NF-κB activation, which may regulate p38α activation and IKKβ-dependent IκBα degradation in response to UV. Consequently, repression of p38α by miR-125b prohibits prolonged hyperactivation of p38α by UV radiation, which is required for protecting cells from UV-induced apoptosis. Altogether, our data support a critical role of NF-κB-dependent up-regulation of miR-125b, which forms a negative feedback loop to repress p38α activation and promote cell survival upon UV radiation.",
author = "Guangyun Tan and Jixiao Niu and Yuling Shi and Hongsheng Ouyang and Zhaohui Wu",
year = "2012",
month = "9",
day = "21",
doi = "10.1074/jbc.M112.383273",
language = "English (US)",
volume = "287",
pages = "33036--33047",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "39",

}

TY - JOUR

T1 - NF-κB-dependent MicroRNA-125b up-regulation promotes cell survival by targeting p38α upon ultraviolet radiation

AU - Tan, Guangyun

AU - Niu, Jixiao

AU - Shi, Yuling

AU - Ouyang, Hongsheng

AU - Wu, Zhaohui

PY - 2012/9/21

Y1 - 2012/9/21

N2 - UV-induced stress response involves expression change of a myriad of genes, which play critical roles in modulating cell cycle arrest, DNA repair, and cell survival. Alteration of micro-RNAs has been found in cells exposed to UV, yet their function in UV stress response remains elusive. Here, we show that UV radiation induces up-regulation of miR-125b, which negatively regulates p38α expression through targeting its 3′-UTR. Increase of miR-125b depends on UV-induced NF-κB activation, which enhances miR-125b gene transcription upon UV radiation. The DNA damage-responsive kinase ATM(ataxia telangiectasia mutated) is indispensable for UV-induced NF-κB activation, which may regulate p38α activation and IKKβ-dependent IκBα degradation in response to UV. Consequently, repression of p38α by miR-125b prohibits prolonged hyperactivation of p38α by UV radiation, which is required for protecting cells from UV-induced apoptosis. Altogether, our data support a critical role of NF-κB-dependent up-regulation of miR-125b, which forms a negative feedback loop to repress p38α activation and promote cell survival upon UV radiation.

AB - UV-induced stress response involves expression change of a myriad of genes, which play critical roles in modulating cell cycle arrest, DNA repair, and cell survival. Alteration of micro-RNAs has been found in cells exposed to UV, yet their function in UV stress response remains elusive. Here, we show that UV radiation induces up-regulation of miR-125b, which negatively regulates p38α expression through targeting its 3′-UTR. Increase of miR-125b depends on UV-induced NF-κB activation, which enhances miR-125b gene transcription upon UV radiation. The DNA damage-responsive kinase ATM(ataxia telangiectasia mutated) is indispensable for UV-induced NF-κB activation, which may regulate p38α activation and IKKβ-dependent IκBα degradation in response to UV. Consequently, repression of p38α by miR-125b prohibits prolonged hyperactivation of p38α by UV radiation, which is required for protecting cells from UV-induced apoptosis. Altogether, our data support a critical role of NF-κB-dependent up-regulation of miR-125b, which forms a negative feedback loop to repress p38α activation and promote cell survival upon UV radiation.

UR - http://www.scopus.com/inward/record.url?scp=84866550126&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84866550126&partnerID=8YFLogxK

U2 - 10.1074/jbc.M112.383273

DO - 10.1074/jbc.M112.383273

M3 - Article

VL - 287

SP - 33036

EP - 33047

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 39

ER -