NF-κB mediates FGF signal regulation of msx-1 expression

Paul B. Bushdid, Chih Li Chen, Dana M. Brantley, Fiona Yull, Rajendra Raghow, Lawrence D. Kerr, Joey V. Barnett

Research output: Contribution to journalArticle

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Abstract

The nuclear factor-κB (NF-κB) family of transcription factors is involved in proliferation, differentiation, and apoptosis in a stage- and cell-dependent manner. Recent evidence has shown that NF-κB activity is necessary for both chicken and mouse limb development. We report here that the NF-κB family member c-rel and the homeodomain gene msx-1 have partially overlapping expression patterns in the developing chick limb. In addition, inhibition of NF-κB activity resulted in a decrease in msx-1 mRNA expression. Sequence analysis of the msx-1 promoter revealed three potential κB-binding sites similar to the interferon-γ (IFN-γ) κB-binding site. These sites bound to c-Rel, as shown by electrophoretic mobility shift assay (EMSA). Furthermore, inhibition of NF-κB activity significantly reduced transactivation of the msx-1 promoter in response to FGF-2/-4, known stimulators of msx-1 expression. These results suggest that NF-κB mediates the FGF-2/-4 signal regulation of msx-1 gene expression.

Original languageEnglish (US)
Pages (from-to)107-115
Number of pages9
JournalDevelopmental Biology
Volume237
Issue number1
DOIs
StatePublished - Sep 1 2001

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Fibroblast Growth Factor 2
rel Genes
Extremities
Binding Sites
Electrophoretic Mobility Shift Assay
Interferons
Transcriptional Activation
Sequence Analysis
Chickens
Transcription Factors
Apoptosis
Gene Expression
Messenger RNA
Inhibition (Psychology)

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Bushdid, P. B., Chen, C. L., Brantley, D. M., Yull, F., Raghow, R., Kerr, L. D., & Barnett, J. V. (2001). NF-κB mediates FGF signal regulation of msx-1 expression. Developmental Biology, 237(1), 107-115. https://doi.org/10.1006/dbio.2001.0356

NF-κB mediates FGF signal regulation of msx-1 expression. / Bushdid, Paul B.; Chen, Chih Li; Brantley, Dana M.; Yull, Fiona; Raghow, Rajendra; Kerr, Lawrence D.; Barnett, Joey V.

In: Developmental Biology, Vol. 237, No. 1, 01.09.2001, p. 107-115.

Research output: Contribution to journalArticle

Bushdid, PB, Chen, CL, Brantley, DM, Yull, F, Raghow, R, Kerr, LD & Barnett, JV 2001, 'NF-κB mediates FGF signal regulation of msx-1 expression', Developmental Biology, vol. 237, no. 1, pp. 107-115. https://doi.org/10.1006/dbio.2001.0356
Bushdid PB, Chen CL, Brantley DM, Yull F, Raghow R, Kerr LD et al. NF-κB mediates FGF signal regulation of msx-1 expression. Developmental Biology. 2001 Sep 1;237(1):107-115. https://doi.org/10.1006/dbio.2001.0356
Bushdid, Paul B. ; Chen, Chih Li ; Brantley, Dana M. ; Yull, Fiona ; Raghow, Rajendra ; Kerr, Lawrence D. ; Barnett, Joey V. / NF-κB mediates FGF signal regulation of msx-1 expression. In: Developmental Biology. 2001 ; Vol. 237, No. 1. pp. 107-115.
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