Nicotine up-regulates expression of orexin and its receptors in rat brain

J. K. Kane, S. L. Parker, S. G. Matta, Y. Fu, Burt Sharp, M. D. Li

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

Orexins are two recently discovered neuropeptides that can stimulate food intake. As the chronic use of tobacco typically leads to a reduction in body weight, it is of interest to determine whether nicotine, the major biologically active tobacco ingredient, has an effect on orexin metabolism in the brain. Using a semiquantitative RT-PCR technique, the levels of messenger RNA (mRNA) for prepro-orexin, orexin A (OX1-R) and orexin B (OX2-R) receptors were 20-50% higher in rats receiving nicotine for 14 days at the level of 2-4 mg/kg day compared with rats receiving saline solvent alone. In animals treated with nicotine at 4 mg/kg day, the expression levels of mRNA for prepro-orexin, OX1-R, and OX2-R were significantly higher compared with those in either the free-feeding control or pair-fed saline control rats. RIA data indicated that both orexin A and orexin B peptide levels were significantly elevated (45-54%; P < 0.01) in the dorsomedial nucleus (DMH) of the nicotine-treated rats compared with either solvent-only or pair-fed controls. Additionally, orexin B was significantly elevated (83%; P < 0.01), over levels in both types of the control animals, in the paraventricular nucleus (PVN) region. In summary, we demonstrated that an inverse association between nicotine and food intake as well as body weight held with doses comparable to those consumed by average human smokers. Moreover, our data indicated that chronic exposure to nicotine can induce a long-term increase in the expression levels of prepro-orexin and their receptor mRNA in the rat hypothalamus and in the levels of orexin A in the DMH and orexin B in the DMH and PVN among the six hypothalamic regions that we examined.

Original languageEnglish (US)
Pages (from-to)3623-3629
Number of pages7
JournalEndocrinology
Volume141
Issue number10
DOIs
StatePublished - Jan 1 2000

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Orexin Receptors
Nicotine
Up-Regulation
Dimenhydrinate
Brain
Paraventricular Hypothalamic Nucleus
Messenger RNA
Eating
Body Weight
Mediodorsal Thalamic Nucleus
Orexins
Tobacco Use
Neuropeptides
Hypothalamus
Tobacco
Polymerase Chain Reaction
Peptides

All Science Journal Classification (ASJC) codes

  • Endocrinology

Cite this

Kane, J. K., Parker, S. L., Matta, S. G., Fu, Y., Sharp, B., & Li, M. D. (2000). Nicotine up-regulates expression of orexin and its receptors in rat brain. Endocrinology, 141(10), 3623-3629. https://doi.org/10.1210/endo.141.10.7707

Nicotine up-regulates expression of orexin and its receptors in rat brain. / Kane, J. K.; Parker, S. L.; Matta, S. G.; Fu, Y.; Sharp, Burt; Li, M. D.

In: Endocrinology, Vol. 141, No. 10, 01.01.2000, p. 3623-3629.

Research output: Contribution to journalArticle

Kane, JK, Parker, SL, Matta, SG, Fu, Y, Sharp, B & Li, MD 2000, 'Nicotine up-regulates expression of orexin and its receptors in rat brain', Endocrinology, vol. 141, no. 10, pp. 3623-3629. https://doi.org/10.1210/endo.141.10.7707
Kane, J. K. ; Parker, S. L. ; Matta, S. G. ; Fu, Y. ; Sharp, Burt ; Li, M. D. / Nicotine up-regulates expression of orexin and its receptors in rat brain. In: Endocrinology. 2000 ; Vol. 141, No. 10. pp. 3623-3629.
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