Nicotinic acetylcholine receptor-mediated synaptic potentials in rat neocortex

Z. G. Chu, Fuming Zhou, J. J. Hablitz

Research output: Contribution to journalArticle

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Abstract

In the neocortex, fast excitatory synaptic transmission can typically be blocked by using excitatory amino acid (EAA) receptor antagonists. In recordings from layer II/III neocortical pyramidal neurons, we observed an evoked excitatory postsynaptic potential (EPSP) or current (EPSC) in the presence of EAA receptor antagonists (40-100 μM D-APV+20 μM CNQX, or 5 mM kynurenic acid) plus the GABAA-receptor antagonist bicuculline (BIC, 20 μM). This EAA-antagonist resistant EPSC was observed in about 70% of neurons tested. It had a duration of approximately 20 ms and an amplitude of 61.5±6.8 pA at -70 mV (n=35). The EAA-antagonist resistant EPSC current-voltage relation was linear and reversed near 0 mV (n=23). The nonselective nicotinic acetylcholine receptor (nAChR) antagonists dihydro-β-erythroidine (DHβE, 100 μM) or mecamylamine (50 μM) reduced EPSC amplitudes by 42 (n=20) and 33% (n=9), respectively. EPSC kinetics were not significantly changed by either antagonist. Bath application of 10 μM neostigmine, a potent acetylcholinesterase inhibitor, prolonged the EPSC decay time. EAA-antagonist resistant EPSCs were observed in the presence of antagonists of metabotropic glutamate, serotonergic (5-HT3) and purinergic (P2) receptors. The EAA-antagonist resistant EPSC appears to be due in part to activation of postsynaptic nAChRs. These results suggest the existence of functional synaptic nAChRs on pyramidal neurons in rat neocortex.

Original languageEnglish (US)
Pages (from-to)399-405
Number of pages7
JournalBrain Research
Volume887
Issue number2
DOIs
StatePublished - Dec 29 2000

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Synaptic Potentials
Excitatory Postsynaptic Potentials
Neocortex
Excitatory Amino Acid Antagonists
Nicotinic Receptors
Pyramidal Cells
Glutamate Receptors
imidazole mustard
Kynurenic Acid
6-Cyano-7-nitroquinoxaline-2,3-dione
Mecamylamine
Purinergic P2 Receptors
GABA-A Receptor Antagonists
Neostigmine
Bicuculline
Cholinesterase Inhibitors
Cholinergic Antagonists
Baths
Synaptic Transmission
Neurons

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Nicotinic acetylcholine receptor-mediated synaptic potentials in rat neocortex. / Chu, Z. G.; Zhou, Fuming; Hablitz, J. J.

In: Brain Research, Vol. 887, No. 2, 29.12.2000, p. 399-405.

Research output: Contribution to journalArticle

Chu, Z. G. ; Zhou, Fuming ; Hablitz, J. J. / Nicotinic acetylcholine receptor-mediated synaptic potentials in rat neocortex. In: Brain Research. 2000 ; Vol. 887, No. 2. pp. 399-405.
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