Nitric oxide attenuates IGF-I-induced aortic smooth muscle cell motility by decreasing Rac1 activity

Essential role of PTP-PEST and p130cas

Alice Corina Ceacareanu, Bogdan Ceacareanu, Daming Zhuang, Yingzi Chang, Ramesh M. Ray, Leena Desai, Kenneth E. Chapman, Christopher Waters, Aviv Hassid

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Recent data support the hypothesis that reactive oxygen species (ROS) play a central role in the initiation and progression of vascular diseases. An important vasoprotective function related to the regulation of ROS levels appears to be the antioxidant capacity of nitric oxide (NO). We previously reported that treatment with NO decreases phosphotyrosine levels of adapter protein p130cas by increasing protein tyrosine phosphatase-proline, glutamate, serine, and threonine sequence protein (PTP-PEST) activity, which leads to the suppression of agonist-induced H2O2 elevation and motility in cultured rat aortic smooth muscle cells (SMCs). The present study was performed to investigate the hypotheses that 1) IGF-I increases the activity of the small GTPase Rac1 as well as H2O2 levels and 2) NO suppresses IGF-I-induced H2O2 elevation by decreasing Rac1 activity via increased PTP-PEST activity and dephosphorylation of p130cas. We report that IGF-I induces phosphorylation of p130 cas and activation of Rac1 and that NO attenuates these effects. The effects of NO are mimicked by the overexpression of PTP-PEST or dominant-negative (dn)-p130cas and antagonized by the expression of dn-PTP-PEST or p130cas. We conclude that IGF-I induces rat aortic SMC motility by increasing phosphotyrosine levels of p130cas and activating Rac1 and that NO decreases motility by activating PTP-PEST, inducing dephosphorylating p130cas, and decreasing Rac1 activity. Decreased Rac1 activity lowers intracellular H2O2 levels, thus attenuating cell motility.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume290
Issue number4
DOIs
StatePublished - Apr 1 2006

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Crk-Associated Substrate Protein
Protein Tyrosine Phosphatases
Threonine
Insulin-Like Growth Factor I
Proline
Serine
Smooth Muscle Myocytes
Cell Movement
Glutamic Acid
Nitric Oxide
Phosphotyrosine
Reactive Oxygen Species
Proteins
Molecular Motor Proteins
Monomeric GTP-Binding Proteins
Vascular Diseases
Antioxidants
Phosphorylation

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cell Biology

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Nitric oxide attenuates IGF-I-induced aortic smooth muscle cell motility by decreasing Rac1 activity : Essential role of PTP-PEST and p130cas. / Ceacareanu, Alice Corina; Ceacareanu, Bogdan; Zhuang, Daming; Chang, Yingzi; Ray, Ramesh M.; Desai, Leena; Chapman, Kenneth E.; Waters, Christopher; Hassid, Aviv.

In: American Journal of Physiology - Cell Physiology, Vol. 290, No. 4, 01.04.2006.

Research output: Contribution to journalArticle

Ceacareanu, Alice Corina ; Ceacareanu, Bogdan ; Zhuang, Daming ; Chang, Yingzi ; Ray, Ramesh M. ; Desai, Leena ; Chapman, Kenneth E. ; Waters, Christopher ; Hassid, Aviv. / Nitric oxide attenuates IGF-I-induced aortic smooth muscle cell motility by decreasing Rac1 activity : Essential role of PTP-PEST and p130cas. In: American Journal of Physiology - Cell Physiology. 2006 ; Vol. 290, No. 4.
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