Nitric oxide

The 'second messenger' of insulin

Nighat N. Kahn, K. Acharya, Sujoy Bhattacharya, R. Acharya, S. Mazumder, William A. Bauman, Asru K. Sinha

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Incubation of various tissues, including heart, liver, kidney, muscle, and intestine from mice and erythrocytes or their membrane fractions from humans, with physiologic concentration of insulin resulted in the activation of a membrane-bound nitric oxide synthase (NOS). Activation of NOS and synthesis of NO were stimulated by the binding of insulin to specific receptors on the cell surface. A Lineweaver-Burk plot of the enzymatic activity demonstrated that the stimulation of NOS by insulin was related to the decrease in the K(m) for L-arginine, the substrate for NOS, with a simultaneous increase of V(max). Addition of N(G)-nitro-L-arginine methyl ester (LNAME), a competitive inhibitor of NOS, to the reaction mixture completely inhibited the hormone-stimulated NO synthesis in all tissues. Furthermore, NO had an insulin-like effect in stimulating glucose transport and glucose oxidation in muscle, a major site for insulin action. Addition of NAME to the reaction mixture completely blocked the stimulatory effect of insulin by inhibiting both NO production and glucose metabolism, without affecting the hormone-stimulated tyrosine or phosphalidylinositol 3-kinases of the membrane preparation. Injection of NO in alloxan-induced diabetic mice mimicked the effect of insulin in the control of hyperglycemia (i.e., lowered the glucose content in plasma). However, injection of NAME before the administration of insulin to diabetic-induced and nondiabetic mice inhibited not only the insulin-stimulated increase of NO in plasma but also the glucose-lowering effect of insulin.

Original languageEnglish (US)
Pages (from-to)441-450
Number of pages10
JournalIUBMB Life
Volume49
Issue number5
StatePublished - Jul 17 2000

Fingerprint

Second Messenger Systems
Nitric Oxide
Insulin
Nitric Oxide Synthase
Glucose
Membranes
Muscle
Chemical activation
Hormones
Tissue
Plasmas
Muscles
Alloxan
Injections
NG-Nitroarginine Methyl Ester
Cell Surface Receptors
Metabolism
Hyperglycemia
Liver
Intestines

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Clinical Biochemistry
  • Cell Biology

Cite this

Kahn, N. N., Acharya, K., Bhattacharya, S., Acharya, R., Mazumder, S., Bauman, W. A., & Sinha, A. K. (2000). Nitric oxide: The 'second messenger' of insulin. IUBMB Life, 49(5), 441-450.

Nitric oxide : The 'second messenger' of insulin. / Kahn, Nighat N.; Acharya, K.; Bhattacharya, Sujoy; Acharya, R.; Mazumder, S.; Bauman, William A.; Sinha, Asru K.

In: IUBMB Life, Vol. 49, No. 5, 17.07.2000, p. 441-450.

Research output: Contribution to journalArticle

Kahn, NN, Acharya, K, Bhattacharya, S, Acharya, R, Mazumder, S, Bauman, WA & Sinha, AK 2000, 'Nitric oxide: The 'second messenger' of insulin', IUBMB Life, vol. 49, no. 5, pp. 441-450.
Kahn NN, Acharya K, Bhattacharya S, Acharya R, Mazumder S, Bauman WA et al. Nitric oxide: The 'second messenger' of insulin. IUBMB Life. 2000 Jul 17;49(5):441-450.
Kahn, Nighat N. ; Acharya, K. ; Bhattacharya, Sujoy ; Acharya, R. ; Mazumder, S. ; Bauman, William A. ; Sinha, Asru K. / Nitric oxide : The 'second messenger' of insulin. In: IUBMB Life. 2000 ; Vol. 49, No. 5. pp. 441-450.
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