Nitrogen catabolite repression of arginase (CAR1) expression in Saccharomyces cerevisiae is derived from regulated inducer exclusion

Terrance Cooper, L. Kovari, R. A. Sumrada, H. D. Park, R. M. Luche, I. Kovari

Research output: Contribution to journalArticle

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Abstract

Expression of the Saccharomyces cerevisiae arginase (CAR1) gene is regulated by induction and nitrogen catabolite repression (NCR). Arginine was demonstrated to be the native inducer. CAR1 sensitivity to NCR has long been accepted to be accomplished through a negative control mechanism, and cis- acting sites for it have been hypothesized. In search of this negatively acting site, we discovered that CAR1 sensitivity to NCR derives from regulated inducer (arginine) exclusion. The route of catabolic entry of arginine into the cell, the general amino acid permease (GAP1), is sensitive to NCR. However, CAR1 expression in the presence of sufficient intracellular arginine is NCR insensitive.

Original languageEnglish (US)
Pages (from-to)48-55
Number of pages8
JournalJournal of Bacteriology
Volume174
Issue number1
DOIs
StatePublished - Jan 1 1992

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Catabolite Repression
Arginase
Saccharomyces cerevisiae
Nitrogen
Arginine
Amino Acid Transport Systems
Genes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Nitrogen catabolite repression of arginase (CAR1) expression in Saccharomyces cerevisiae is derived from regulated inducer exclusion. / Cooper, Terrance; Kovari, L.; Sumrada, R. A.; Park, H. D.; Luche, R. M.; Kovari, I.

In: Journal of Bacteriology, Vol. 174, No. 1, 01.01.1992, p. 48-55.

Research output: Contribution to journalArticle

Cooper, Terrance ; Kovari, L. ; Sumrada, R. A. ; Park, H. D. ; Luche, R. M. ; Kovari, I. / Nitrogen catabolite repression of arginase (CAR1) expression in Saccharomyces cerevisiae is derived from regulated inducer exclusion. In: Journal of Bacteriology. 1992 ; Vol. 174, No. 1. pp. 48-55.
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