Nitrogen catabolite repression-sensitive transcription as a readout of Tor pathway regulation

The genetic background, reporter gene and GATA factor assayed determine the outcomes

Isabelle Georis, André Feller, Jennifer J. Tate, Terrance Cooper, Evelyne Dubois

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Nitrogen catabolite repression (NCR)-sensitive genes, whose expression is highly repressed when provided with excess nitrogen and derepressed when nitrogen is limited or cells are treated with rapainycin, are routinely used as reporters in mechanistic studies of the Tor signal transduction pathway in Saccharomyces cerevisiae. Two GATA factors, Gln3 and Gat1, are responsible for NCR-sensitive transcription, but recent evidence demonstrates that Tor pathway regulation of NCR-sensitive transcription bifurcates at the level of GATA factor localization. Gin3 requires Sit4 phosphatase for nuclear localization and NCR-sensitive transcription while Gatl does not. In this article, we demonstrate that the extent to which Sit4 plays a role in NCR-sensitive transcription depends upon whether or not (i) Gzf3, a GATA repressor homologous to Dal80, is active in the genetic background assayed: (ii) Gat1 is able to activate transcription of the assayed gene in the absence of Gln3 in that genetic background; and (iii) the gene chosen as a reporter is able to be transcribed by Gln3 or Gat1 in the absence of the other GATA factor. Together, the data indicate that in the absence of these three pieces of information, overall NCR-sensitive gene transcription data are unreliable as Tor pathway readouts.

Original languageEnglish (US)
Pages (from-to)861-874
Number of pages14
JournalGenetics
Volume181
Issue number3
DOIs
StatePublished - Mar 1 2009

Fingerprint

GATA Transcription Factors
Catabolite Repression
Reporter Genes
Nitrogen
Genes
Genetic Background
Phosphoric Monoester Hydrolases
Saccharomyces cerevisiae
Signal Transduction
Gene Expression

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Nitrogen catabolite repression-sensitive transcription as a readout of Tor pathway regulation : The genetic background, reporter gene and GATA factor assayed determine the outcomes. / Georis, Isabelle; Feller, André; Tate, Jennifer J.; Cooper, Terrance; Dubois, Evelyne.

In: Genetics, Vol. 181, No. 3, 01.03.2009, p. 861-874.

Research output: Contribution to journalArticle

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