Non-Invasive Detection of Lung Inflammation by Near-Infrared Fluorescence Imaging Using Bimodal Liposomes

Hari R. Desu, George C. Wood, Laura Thoma

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Acute lung injury (ALI) and its more severe form, acute respiratory distress syndrome results in respiratory obstruction and severe lung inflammation. Critical characteristics of ALI are alveolar edema, infiltration of leukocytes (neutrophils and monocytes), release of pro-inflammatory cytokines and chemokines into broncho-alveolar lavage fluid, and activation of integrin receptors. The purpose of the study was to demonstrate non-invasive detection of lung inflammation using integrin receptor targeted fluorescence liposomes. An inflammation similar to that observed in ALI was elicited in rodents by intra-tracheal instillation of interleukin-1beta (IL-1beta). Cyclic arginine glycine-(D)-aspartic acid-peptide (cRGD-peptide) grafted fluorescence liposomes were administered to ALI induced male Sprague-Dawley rats for targeting lung integrin receptors. Near-infrared fluorescence imaging (NIRFI) was applied for visualization and quantitation of lung inflammation. NIRFI signals were correlated with inflammatory cellular and biochemical markers of lungs. A positive correlation was observed between NIRF signals and lung inflammation markers. Compared to control group, an intense NIRF signal was observed in ALI induced rats in the window 6-24 h post-IL-1beta instillation. Interaction of integrin receptors with targeted liposomes was assumed to contribute to intense NIRF signal. RT-PCR studies showed an elevated lung expression of alphavbeta5 integrin receptors, 12 h post-IL-1beta instillation. In vitro studies demonstrated integrin receptor specificity of targeted liposomes. These targeted liposomes showed binding to alphavbeta5 integrin receptors expressed on alveolar cells. Non-invasive detection of lung inflammation was demonstrated using a combination of integrin receptor targeting and NIRFI.

Original languageEnglish (US)
Pages (from-to)241-253
Number of pages13
JournalJournal of Fluorescence
Volume26
Issue number1
DOIs
StatePublished - Jan 1 2016

Fingerprint

Optical Imaging
subversion
Integrins
Liposomes
Acute Lung Injury
activation
visualization
Pneumonia
Fluorescence
Infrared radiation
Imaging techniques
interaction
Interleukin-1beta
Group
Lung
Rats
Alveolar Epithelial Cells
D-Aspartic Acid
Neutrophil Infiltration
Adult Respiratory Distress Syndrome

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Psychology
  • Social Sciences (miscellaneous)
  • Sociology and Political Science
  • Spectroscopy
  • Clinical Biochemistry
  • Law

Cite this

Non-Invasive Detection of Lung Inflammation by Near-Infrared Fluorescence Imaging Using Bimodal Liposomes. / Desu, Hari R.; Wood, George C.; Thoma, Laura.

In: Journal of Fluorescence, Vol. 26, No. 1, 01.01.2016, p. 241-253.

Research output: Contribution to journalArticle

Desu, Hari R. ; Wood, George C. ; Thoma, Laura. / Non-Invasive Detection of Lung Inflammation by Near-Infrared Fluorescence Imaging Using Bimodal Liposomes. In: Journal of Fluorescence. 2016 ; Vol. 26, No. 1. pp. 241-253.
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