Non-invasive dual fluorescence in vivo imaging for detection of macrophage infiltration and matrix metalloproteinase (MMP) activity in inflammatory arthritic joints

Hongsik Cho, Fazal Ur Rehman Bhatti, Tae Won Yoon, Karen Hasty, John Stuart, Ae-Kyung Yi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Detection and intervention at an early stage is a critical factor to impede arthritis progress. Here we present a non-invasive method to detect inflammatory changes in joints of arthritic mice. Inflammation was monitored by dual fluorescence optical imaging for near-infrared fluorescent (750F) matrix-metalloproteinase activatable agent and allophycocyanin-conjugated anti-mouse CD11b. Increased intensity of allophycocyanin (indication of macrophage accumulation) and 750F (indication of matrix-metalloproteinase activity) showed a biological relationship with the arthritis severity score and the histopathology score of arthritic joints. Our results demonstrate that this method can be used to detect early stages of arthritis with minimum intervention in small animal models.

Original languageEnglish (US)
Pages (from-to)1842-1852
Number of pages11
JournalBiomedical Optics Express
Volume7
Issue number5
DOIs
StatePublished - May 1 2016

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arthritis
macrophages
infiltration
Matrix Metalloproteinases
Arthritis
Joints
Fluorescence
Macrophages
fluorescence
mice
indication
matrices
Optical Imaging
animal models
Animal Models
Inflammation

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Cite this

Non-invasive dual fluorescence in vivo imaging for detection of macrophage infiltration and matrix metalloproteinase (MMP) activity in inflammatory arthritic joints. / Cho, Hongsik; Bhatti, Fazal Ur Rehman; Yoon, Tae Won; Hasty, Karen; Stuart, John; Yi, Ae-Kyung.

In: Biomedical Optics Express, Vol. 7, No. 5, 01.05.2016, p. 1842-1852.

Research output: Contribution to journalArticle

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