Nonsense suppression in Schizosaccharomyces pombe

The S. pombe Sup3-e tRNASer UGA gene is active in S. cerevisiae

Herbert Hottinger, David Pearson, Fumiaki Yamao, Vera Gamulin, Lynn Colley, Terrance Cooper, Dieter Söll

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The gene encoding the efficient UGA suppressor sup3-e of Schizosaccharomyces pombe was isolated by in vivo transformation of Saccharomyces cerevisiae UGA mutants with S. pombe sup3-e DNA. DNA from a clone bank of EcoRI fragments from a S. pombe sup3-e strain in the hybrid yeast vector YRp17 was used to transform the S. cerevisiae multiple auxotroph his4-260 leu2-2 trp1-1 to prototrophy. Transformants were isolated at a low frequency; they lost the ability to grow in minimal medium after passaging in non-selective media. This suggested the presence of the suppressor gene on the non-integrative plasmid. Plasmid DNA, isolated from the transformed S. cerevisiae cells and subsequently amplified in E. coli, transformed S. cerevisiae his4-260 leu2-2 trp1-1 to prototrophy. In this way a 2.4 kb S. pombe DNA fragment carrying the sup3-e gene was isolated. Sequence analysis revealed the presence of two tRNA coding regions separated by a spacer of only seven nucleotides. The sup3-e tRNASer UGAtRNA gene is followed by a sequence coding for the initiator tRNAMet. The transformation results demonstrate that the cloned S. pombe UGA suppressor is active in S. cerevisiae UGA mutant strains.

Original languageEnglish (US)
Pages (from-to)219-224
Number of pages6
JournalMGG Molecular & General Genetics
Volume188
Issue number2
DOIs
StatePublished - Dec 1 1982
Externally publishedYes

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RNA, Transfer, Ser
Schizosaccharomyces
Saccharomyces cerevisiae
Genes
DNA
Plasmids
RNA, Transfer, Met
Suppressor Genes
Transfer RNA
Sequence Analysis
Nucleotides
Clone Cells
Yeasts
Escherichia coli

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Nonsense suppression in Schizosaccharomyces pombe : The S. pombe Sup3-e tRNASer UGA gene is active in S. cerevisiae. / Hottinger, Herbert; Pearson, David; Yamao, Fumiaki; Gamulin, Vera; Colley, Lynn; Cooper, Terrance; Söll, Dieter.

In: MGG Molecular & General Genetics, Vol. 188, No. 2, 01.12.1982, p. 219-224.

Research output: Contribution to journalArticle

Hottinger, Herbert ; Pearson, David ; Yamao, Fumiaki ; Gamulin, Vera ; Colley, Lynn ; Cooper, Terrance ; Söll, Dieter. / Nonsense suppression in Schizosaccharomyces pombe : The S. pombe Sup3-e tRNASer UGA gene is active in S. cerevisiae. In: MGG Molecular & General Genetics. 1982 ; Vol. 188, No. 2. pp. 219-224.
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