Noradrenergic stimulation of supraoptic neuronal activity and vasopressin release in vitro

mediation by an α1-receptor

William Armstrong, Mark J. Gallagher, Celia D. Sladek

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Using the isolated rat hypothalamoneurohypophysial system, the effects of noradrenaline (NA) and some adrenoceptor agonists on vasopressin (VP) release and supraoptic neuronal activity were assessed in vitro. Extracellular action potentials driven antidromically by neural stalk stimulation were recorded while fractions of the perifusate were collected every minute for radioimmunoassay of VP. NA and the α1-agonist phenylephrine stimulated VP release and elicited bursts in tuberal supraoptic neurons, whereas isoprenaline (a β-agonist) and clonidine (an α2-agonist) did not. The peak rate of VP release was reached shortly after the onset of burst discharge, when intraburst firing rate was highest, and both effects were dose-dependent when tested to different concentrations of phenylephrine. Thus VP secreting neurons are excited to release VP by α1-adrenoceptor activation.

Original languageEnglish (US)
Pages (from-to)192-197
Number of pages6
JournalBrain Research
Volume365
Issue number1
DOIs
StatePublished - Feb 12 1986

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Vasopressins
Phenylephrine
Adrenergic Receptors
Norepinephrine
Neurons
Clonidine
Isoproterenol
Action Potentials
Radioimmunoassay
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Noradrenergic stimulation of supraoptic neuronal activity and vasopressin release in vitro : mediation by an α1-receptor. / Armstrong, William; Gallagher, Mark J.; Sladek, Celia D.

In: Brain Research, Vol. 365, No. 1, 12.02.1986, p. 192-197.

Research output: Contribution to journalArticle

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