Norepinephrine modulates the inflammatory and proliferate phases of wound healing

Ankush Gosain, Stephen B. Jones, Ravi Shankar, Richard L. Gamelli, Luisa A. DiPietro, Lewis J. Kaplan

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Background: Injury results in the massive release of norepinephrine (NE) into the peripheral circulation. Recent investigations have demonstrated functional adrenoreceptors on the cellular mediators of cutaneous wound healing and NE-induced phenotypic alterations in immune cells have been demonstrated in vitro. Despite this, there is little description of how NE might alter the phases of wound healing in vivo. The purpose of this study was to compare cutaneous wound healing in norepinephrine-intact and norepinephrine-depleted mice. Methods: Norepinephrine-depleted (NED) mice were generated by chemical axotomy with 6-hydroxydopamine and compared with norepinephrine-intact (NEI) animals (n = 6-12 per group, per time point). Using an excisional wound model, neutrophil recruitment was measured by myeloperoxidase assay. Macrophage recruitment and angiogenesis were measured by immunohistochemistry and reepithelialization was determined histologically. The development of incisional wound disruption strength was determined over time. Finally, macrophage scavenger function was assessed by an in vitro latex bead phagocytosis assay. Results: Wounds from NEI mice demonstrated greater neutrophil infiltration than NED wounds (24, 72 hours; p < 0.05). Wound macrophage recruitment was initially higher in NEI animals (24 hours, p < 0.05), but was eventually surpassed by that of NED animals (120 hours, p < 0.05). Angiogenesis was decreased while re-epithelialization was accelerated in NEI animals (p < 0.05). Wound disruption strength and macrophage scavenger function were unaltered between NED and NEI mice. Conclusions: Norepinephrine modulates the inflammatory and proliferative phases of wound healing in a temporally defined, cell-specific manner. By increasing recruitment of innate immune cells and expediting wound closure, norepinephrine appears to play a protective role in defense against infection.

Original languageEnglish (US)
Pages (from-to)736-744
Number of pages9
JournalJournal of Trauma - Injury, Infection and Critical Care
Volume60
Issue number4
DOIs
StatePublished - Apr 1 2006
Externally publishedYes

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Wound Healing
Norepinephrine
Wounds and Injuries
Macrophages
Neutrophil Infiltration
Re-Epithelialization
Axotomy
Skin
Oxidopamine
Microspheres
Phagocytosis
Peroxidase

All Science Journal Classification (ASJC) codes

  • Surgery
  • Critical Care and Intensive Care Medicine

Cite this

Norepinephrine modulates the inflammatory and proliferate phases of wound healing. / Gosain, Ankush; Jones, Stephen B.; Shankar, Ravi; Gamelli, Richard L.; DiPietro, Luisa A.; Kaplan, Lewis J.

In: Journal of Trauma - Injury, Infection and Critical Care, Vol. 60, No. 4, 01.04.2006, p. 736-744.

Research output: Contribution to journalArticle

Gosain, Ankush ; Jones, Stephen B. ; Shankar, Ravi ; Gamelli, Richard L. ; DiPietro, Luisa A. ; Kaplan, Lewis J. / Norepinephrine modulates the inflammatory and proliferate phases of wound healing. In: Journal of Trauma - Injury, Infection and Critical Care. 2006 ; Vol. 60, No. 4. pp. 736-744.
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abstract = "Background: Injury results in the massive release of norepinephrine (NE) into the peripheral circulation. Recent investigations have demonstrated functional adrenoreceptors on the cellular mediators of cutaneous wound healing and NE-induced phenotypic alterations in immune cells have been demonstrated in vitro. Despite this, there is little description of how NE might alter the phases of wound healing in vivo. The purpose of this study was to compare cutaneous wound healing in norepinephrine-intact and norepinephrine-depleted mice. Methods: Norepinephrine-depleted (NED) mice were generated by chemical axotomy with 6-hydroxydopamine and compared with norepinephrine-intact (NEI) animals (n = 6-12 per group, per time point). Using an excisional wound model, neutrophil recruitment was measured by myeloperoxidase assay. Macrophage recruitment and angiogenesis were measured by immunohistochemistry and reepithelialization was determined histologically. The development of incisional wound disruption strength was determined over time. Finally, macrophage scavenger function was assessed by an in vitro latex bead phagocytosis assay. Results: Wounds from NEI mice demonstrated greater neutrophil infiltration than NED wounds (24, 72 hours; p < 0.05). Wound macrophage recruitment was initially higher in NEI animals (24 hours, p < 0.05), but was eventually surpassed by that of NED animals (120 hours, p < 0.05). Angiogenesis was decreased while re-epithelialization was accelerated in NEI animals (p < 0.05). Wound disruption strength and macrophage scavenger function were unaltered between NED and NEI mice. Conclusions: Norepinephrine modulates the inflammatory and proliferative phases of wound healing in a temporally defined, cell-specific manner. By increasing recruitment of innate immune cells and expediting wound closure, norepinephrine appears to play a protective role in defense against infection.",
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AU - Kaplan, Lewis J.

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