Novel piperazine induces apoptosis in U937 cells

Josiah J. Sampson, Isaac Donkor, Tien L. Huang, Samuel E. Adunyah

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The effect of 1,4-bis-(4-(1H-benzo[d]imidazol-2-yl-phenyl)) piperazine (BIPP), a newly synthesized piperazine derivative, on U937 leukemia cell viability was investigated. We show that BIPP induces dose-responsive apoptotic cell death in U937 cells by intrinsic mechanisms of apoptosis. Maximum apoptotic effect of BIPP on U937 cells was observed at 12.8μM. BIPP-induced apoptosis was evident by characteristics such as altered annexin-V binding, caspase activation, loss of mitochondrial membrane potential (MMP) and cytochrome c release. BIPP also differentially activates initiator and effector caspases combined with the loss of MMP strongly suggesting that BIPP causes an intrinsic apoptosis in U937 leukemia cells. Due to our observations that BIPP induces leukemia cell death without significantly affecting normal cells, our data suggests that it may be a potential therapeutic agent for human myeloid leukemia.

Original languageEnglish (US)
Pages (from-to)78-88
Number of pages11
JournalInternational Journal of Biochemistry and Molecular Biology
Volume2
Issue number1
StatePublished - 2011

Fingerprint

U937 Cells
Cell death
Apoptosis
Leukemia
Mitochondrial Membrane Potential
Initiator Caspases
Effector Caspases
Membranes
Cell Death
Annexin A5
Caspases
Cytochromes c
Myeloid Leukemia
Chemical activation
Cells
Derivatives
Cell Survival
piperazine
Therapeutics

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biochemistry

Cite this

Novel piperazine induces apoptosis in U937 cells. / Sampson, Josiah J.; Donkor, Isaac; Huang, Tien L.; Adunyah, Samuel E.

In: International Journal of Biochemistry and Molecular Biology, Vol. 2, No. 1, 2011, p. 78-88.

Research output: Contribution to journalArticle

Sampson, Josiah J. ; Donkor, Isaac ; Huang, Tien L. ; Adunyah, Samuel E. / Novel piperazine induces apoptosis in U937 cells. In: International Journal of Biochemistry and Molecular Biology. 2011 ; Vol. 2, No. 1. pp. 78-88.
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