Novel template-casting technique for fabricating β-tricalcium phosphate scaffolds with high interconnectivity and mechanical strength and in vitro cell responses

Yongxing Liu, Joong Hyun Kim, Daniel Young, Sungwoo Kim, Satoru Nishimoto, Yunzhi Yang

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

A novel template-casting method was developed to produce completely interconnected, macroporous biodegradable b-tricalcium phosphate (β-TCP) scaffolds, whose architecture and chemistry can be fully manipulated by varying the templates and casting materials. The processing route includes preparation of β-TCP slurry; casting and shaping into preformed templates comprised of paraffin beads; solidifying, drying; and sintering. Structural, chemical, and mechanical properties of the prepared macroporous scaffolds were characterized using micro computed tomography, scanning electron microscopy, x-ray diffractometry, Fourier transform infrared spectroscopy, and mechanical testing. Human embryonic palatal mesenchymal cells were used to evaluate cell proliferation within the scaffolds in vitro. The scaffolds consisted of interconnected macropores and solid struts, leading to a reticular network. Two groups of scaffolds with larger pores, approximately 600-800 lm and smaller pores approximately 350-500 μm, were demonstrated. The interconnected windows between neighboring macropores were 440 ± 57 lm in diameter for the larger-pored scaffolds, and 330 ± 50 lm for the smaller-pored scaffolds. The scaffolds were highly crystallized and composed dominantly of b-tricalcium phosphate (β-TCP) accompanied by minor phase of hydroxyapatite (HA). The hydroxyl group was clearly detected by FTIR on the scaffolds. High mechanical strength (9.3 MPa) was demonstrated by the completely interconnected scaffolds with ∼79% porosity. The human embryonic palatal mesenchymal (HEPM) cells proliferated well on the smaller-pored and larger-pored scaffolds, exhibiting a significantly higher level of proliferation in the first 11 days of culture on the smaller pored scaffolds. High levels of differentiation were also evidenced in both pore sizes of scaffolds.

Original languageEnglish (US)
Pages (from-to)997-1006
Number of pages10
JournalJournal of Biomedical Materials Research - Part A
Volume92
Issue number3
DOIs
StatePublished - Mar 1 2010

Fingerprint

Scaffolds
Strength of materials
Casting
Phosphates
tricalcium phosphate
Mechanical testing
Struts
Cell proliferation
Durapatite
Scaffolds (biology)
Hydroxyapatite
Hydroxyl Radical
Paraffin
Paraffins
Chemical properties
Pore size
Fourier transform infrared spectroscopy
Tomography
Structural properties
Drying

All Science Journal Classification (ASJC) codes

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

Cite this

Novel template-casting technique for fabricating β-tricalcium phosphate scaffolds with high interconnectivity and mechanical strength and in vitro cell responses. / Liu, Yongxing; Kim, Joong Hyun; Young, Daniel; Kim, Sungwoo; Nishimoto, Satoru; Yang, Yunzhi.

In: Journal of Biomedical Materials Research - Part A, Vol. 92, No. 3, 01.03.2010, p. 997-1006.

Research output: Contribution to journalArticle

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